Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)

T. Toojinda; L. H. Broers; X. M. Chen; P. M. Hayes; A. Kleinhofs; J. Korte; D. Kudrna; H. Leung; R. F. Line; W. Powell; L. Ramsay; H. Vivar; R. Waugh

doi:10.1007/s001220051519

Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)

T. Toojinda, L. H. Broers, X. M. Chen, P. M. Hayes (Lead / Corresponding author), A. Kleinhofs, J. Korte, D. Kudrna, H. Leung, R. F. Line, W. Powell, L. Ramsay, H. Vivar, R. Waugh

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

Stripe rust, leaf rust, and Barley Yellow Dwarf Virus (BYDV) are important diseases of barley (Hordeum vulgare L). Using 94 doubled-haploid lines (DH) from the cross of Shyri x Galena, multiple disease phenotype datasets, and a 99-marker linkage map, we determined the number, genome location, and effects of genes conferring resistance to these diseases. We also mapped Resistance Gene Analog Polymorphism (RGAP) loci, based on degenerate motifs of cloned disease resistance genes, in the same population. Leaf rust resistance was determined by a single gene on chromosome 1 (7H). QTLs on chromosomes 2 (2H), 3 (3H), 5 (1H), and 6 (6H) were the principal determinants of resistance to stripe rust. Two-locus QTL interactions were significant determinants of resistance to this disease. Resistance to the MAV and PAV serotypes of BYDV was determined by coincident QTLs on chromosomes 1 (7H), 4 (4H), and 5 (1H). QTL interactions were not significant for BYDV resistance. The associations of molecular markers with qualitative and quantitative disease resistance loci will be a useful information for marker-assisted selection.

Original language	English
Pages (from-to)	580-589
Number of pages	10
Journal	Theoretical and Applied Genetics
Volume	101
Issue number	4
DOIs	https://doi.org/10.1007/s001220051519
Publication status	Published - 1 Jan 2000

Keywords

Barley
BYDV
Genome mapping
Leaf rust
QTL
Resistance Gene Analog Polymorphism
Stripe rust

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Genetics

Access to Document

10.1007/s001220051519

Cite this

Toojinda, T., Broers, L. H., Chen, X. M., Hayes, P. M., Kleinhofs, A., Korte, J., Kudrna, D., Leung, H., Line, R. F., Powell, W., Ramsay, L., Vivar, H., & Waugh, R. (2000). Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare). Theoretical and Applied Genetics, 101(4), 580-589. https://doi.org/10.1007/s001220051519

@article{6048b8f2223e43788f983fe4545b6031,

title = "Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)",

abstract = "Stripe rust, leaf rust, and Barley Yellow Dwarf Virus (BYDV) are important diseases of barley (Hordeum vulgare L). Using 94 doubled-haploid lines (DH) from the cross of Shyri x Galena, multiple disease phenotype datasets, and a 99-marker linkage map, we determined the number, genome location, and effects of genes conferring resistance to these diseases. We also mapped Resistance Gene Analog Polymorphism (RGAP) loci, based on degenerate motifs of cloned disease resistance genes, in the same population. Leaf rust resistance was determined by a single gene on chromosome 1 (7H). QTLs on chromosomes 2 (2H), 3 (3H), 5 (1H), and 6 (6H) were the principal determinants of resistance to stripe rust. Two-locus QTL interactions were significant determinants of resistance to this disease. Resistance to the MAV and PAV serotypes of BYDV was determined by coincident QTLs on chromosomes 1 (7H), 4 (4H), and 5 (1H). QTL interactions were not significant for BYDV resistance. The associations of molecular markers with qualitative and quantitative disease resistance loci will be a useful information for marker-assisted selection.",

keywords = "Barley, BYDV, Genome mapping, Leaf rust, QTL, Resistance Gene Analog Polymorphism, Stripe rust",

author = "T. Toojinda and Broers, {L. H.} and Chen, {X. M.} and Hayes, {P. M.} and A. Kleinhofs and J. Korte and D. Kudrna and H. Leung and Line, {R. F.} and W. Powell and L. Ramsay and H. Vivar and R. Waugh",

year = "2000",

month = jan,

day = "1",

doi = "10.1007/s001220051519",

language = "English",

volume = "101",

pages = "580--589",

journal = "Theoretical and Applied Genetics",

issn = "0040-5752",

publisher = "Springer Verlag",

number = "4",

}

Toojinda, T, Broers, LH, Chen, XM, Hayes, PM, Kleinhofs, A, Korte, J, Kudrna, D, Leung, H, Line, RF, Powell, W, Ramsay, L, Vivar, H & Waugh, R 2000, 'Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)', Theoretical and Applied Genetics, vol. 101, no. 4, pp. 580-589. https://doi.org/10.1007/s001220051519

TY - JOUR

T1 - Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)

AU - Toojinda, T.

AU - Broers, L. H.

AU - Chen, X. M.

AU - Hayes, P. M.

AU - Kleinhofs, A.

AU - Korte, J.

AU - Kudrna, D.

AU - Leung, H.

AU - Line, R. F.

AU - Powell, W.

AU - Ramsay, L.

AU - Vivar, H.

AU - Waugh, R.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Stripe rust, leaf rust, and Barley Yellow Dwarf Virus (BYDV) are important diseases of barley (Hordeum vulgare L). Using 94 doubled-haploid lines (DH) from the cross of Shyri x Galena, multiple disease phenotype datasets, and a 99-marker linkage map, we determined the number, genome location, and effects of genes conferring resistance to these diseases. We also mapped Resistance Gene Analog Polymorphism (RGAP) loci, based on degenerate motifs of cloned disease resistance genes, in the same population. Leaf rust resistance was determined by a single gene on chromosome 1 (7H). QTLs on chromosomes 2 (2H), 3 (3H), 5 (1H), and 6 (6H) were the principal determinants of resistance to stripe rust. Two-locus QTL interactions were significant determinants of resistance to this disease. Resistance to the MAV and PAV serotypes of BYDV was determined by coincident QTLs on chromosomes 1 (7H), 4 (4H), and 5 (1H). QTL interactions were not significant for BYDV resistance. The associations of molecular markers with qualitative and quantitative disease resistance loci will be a useful information for marker-assisted selection.

AB - Stripe rust, leaf rust, and Barley Yellow Dwarf Virus (BYDV) are important diseases of barley (Hordeum vulgare L). Using 94 doubled-haploid lines (DH) from the cross of Shyri x Galena, multiple disease phenotype datasets, and a 99-marker linkage map, we determined the number, genome location, and effects of genes conferring resistance to these diseases. We also mapped Resistance Gene Analog Polymorphism (RGAP) loci, based on degenerate motifs of cloned disease resistance genes, in the same population. Leaf rust resistance was determined by a single gene on chromosome 1 (7H). QTLs on chromosomes 2 (2H), 3 (3H), 5 (1H), and 6 (6H) were the principal determinants of resistance to stripe rust. Two-locus QTL interactions were significant determinants of resistance to this disease. Resistance to the MAV and PAV serotypes of BYDV was determined by coincident QTLs on chromosomes 1 (7H), 4 (4H), and 5 (1H). QTL interactions were not significant for BYDV resistance. The associations of molecular markers with qualitative and quantitative disease resistance loci will be a useful information for marker-assisted selection.

KW - Barley

KW - BYDV

KW - Genome mapping

KW - Leaf rust

KW - QTL

KW - Resistance Gene Analog Polymorphism

KW - Stripe rust

UR - http://www.scopus.com/inward/record.url?scp=0033800850&partnerID=8YFLogxK

U2 - 10.1007/s001220051519

DO - 10.1007/s001220051519

M3 - Article

AN - SCOPUS:0033800850

SN - 0040-5752

VL - 101

SP - 580

EP - 589

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

IS - 4

ER -

Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this