Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)

T. Toojinda, L. H. Broers, X. M. Chen, P. M. Hayes (Lead / Corresponding author), A. Kleinhofs, J. Korte, D. Kudrna, H. Leung, R. F. Line, W. Powell, L. Ramsay, H. Vivar, R. Waugh

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Stripe rust, leaf rust, and Barley Yellow Dwarf Virus (BYDV) are important diseases of barley (Hordeum vulgare L). Using 94 doubled-haploid lines (DH) from the cross of Shyri x Galena, multiple disease phenotype datasets, and a 99-marker linkage map, we determined the number, genome location, and effects of genes conferring resistance to these diseases. We also mapped Resistance Gene Analog Polymorphism (RGAP) loci, based on degenerate motifs of cloned disease resistance genes, in the same population. Leaf rust resistance was determined by a single gene on chromosome 1 (7H). QTLs on chromosomes 2 (2H), 3 (3H), 5 (1H), and 6 (6H) were the principal determinants of resistance to stripe rust. Two-locus QTL interactions were significant determinants of resistance to this disease. Resistance to the MAV and PAV serotypes of BYDV was determined by coincident QTLs on chromosomes 1 (7H), 4 (4H), and 5 (1H). QTL interactions were not significant for BYDV resistance. The associations of molecular markers with qualitative and quantitative disease resistance loci will be a useful information for marker-assisted selection.

Original languageEnglish
Pages (from-to)580-589
Number of pages10
JournalTheoretical and Applied Genetics
Volume101
Issue number4
DOIs
Publication statusPublished - 1 Jan 2000

Fingerprint

Disease Resistance
Luteovirus
Haploidy
doubled haploids
Hordeum
Barley yellow dwarf virus
Hordeum vulgare
disease resistance
quantitative trait loci
barley
Chromosomes, Human, Pair 1
leaf rust
chromosomes
Population
loci
Genes
genes
Chromosomes, Human, Pair 2
Virus Diseases
marker-assisted selection

Keywords

  • Barley
  • BYDV
  • Genome mapping
  • Leaf rust
  • QTL
  • Resistance Gene Analog Polymorphism
  • Stripe rust

Cite this

Toojinda, T. ; Broers, L. H. ; Chen, X. M. ; Hayes, P. M. ; Kleinhofs, A. ; Korte, J. ; Kudrna, D. ; Leung, H. ; Line, R. F. ; Powell, W. ; Ramsay, L. ; Vivar, H. ; Waugh, R. / Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare). In: Theoretical and Applied Genetics. 2000 ; Vol. 101, No. 4. pp. 580-589.
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Toojinda, T, Broers, LH, Chen, XM, Hayes, PM, Kleinhofs, A, Korte, J, Kudrna, D, Leung, H, Line, RF, Powell, W, Ramsay, L, Vivar, H & Waugh, R 2000, 'Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)', Theoretical and Applied Genetics, vol. 101, no. 4, pp. 580-589. https://doi.org/10.1007/s001220051519

Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare). / Toojinda, T.; Broers, L. H.; Chen, X. M.; Hayes, P. M. (Lead / Corresponding author); Kleinhofs, A.; Korte, J.; Kudrna, D.; Leung, H.; Line, R. F.; Powell, W.; Ramsay, L.; Vivar, H.; Waugh, R.

In: Theoretical and Applied Genetics, Vol. 101, No. 4, 01.01.2000, p. 580-589.

Research output: Contribution to journalArticle

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T1 - Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)

AU - Toojinda, T.

AU - Broers, L. H.

AU - Chen, X. M.

AU - Hayes, P. M.

AU - Kleinhofs, A.

AU - Korte, J.

AU - Kudrna, D.

AU - Leung, H.

AU - Line, R. F.

AU - Powell, W.

AU - Ramsay, L.

AU - Vivar, H.

AU - Waugh, R.

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