Mapping the H2 resistance effective against Globodera pallida pathotype Pa1 in tetraploid potato

Shona M . Strachan, Miles R. Armstrong, Amanpreet Kaur, Kathryn M. Wright, Tze Yin Lim, John Jones, Glenn J. Bryan, Vivian Blok, Ingo Hein (Lead / Corresponding author)

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Abstract

Key message: The nematode resistance gene H2 was mapped to the distal end of chromosome 5 in tetraploid potato. Abstract: The H2 resistance gene, introduced into cultivated potatoes from the wild diploid species Solanum multidissectum, confers a high level of resistance to the Pa1 pathotype of the potato cyst nematode Globodera pallida. A cross between tetraploid H2-containing breeding clone P55/7 and susceptible potato variety Picasso yielded an F1 population that segregated approximately 1:1 for the resistance phenotype, which is consistent with a single dominant gene in a simplex configuration. Using genome reduction methodologies RenSeq and GenSeq, the segregating F1 population enabled the genetic characterisation of the resistance through a bulked segregant analysis. A diagnostic RenSeq analysis of the parents confirmed that the resistance in P55/7 cannot be explained by previously characterised resistance genes. Only the variety Picasso contained functionally characterised disease resistance genes Rpi-R1, Rpi-R3a, Rpi-R3b variant, Gpa2 and Rx, which was independently confirmed through effector vacuum infiltration assays. RenSeq and GenSeq independently identified sequence polymorphisms linked to the H2 resistance on the top end of potato chromosome 5. Allele-specific KASP markers further defined the locus containing the H2 gene to a 4.7 Mb interval on the distal short arm of potato chromosome 5 and to positions that correspond to 1.4 MB and 6.1 MB in the potato reference genome.

Original languageEnglish
Pages (from-to)1283-1294
Number of pages12
JournalTheoretical and Applied Genetics
Volume132
Issue number4
Early online date21 Jan 2019
DOIs
Publication statusPublished - Apr 2019

Fingerprint

Globodera pallida
Tetraploidy
pathotypes
Solanum tuberosum
tetraploidy
potatoes
Chromosomes, Human, Pair 5
Genes
Solanum candolleanum
chromosomes
genes
Genome
Solanum
Dominant Genes
Disease Resistance
genome
cyst nematodes
Population Genetics
dominant genes
Vacuum

Keywords

  • Globodera pallida
  • H2
  • tetraploid potato
  • RenSeq
  • dRenSeq
  • GenSeq

Cite this

Strachan, Shona M . ; Armstrong, Miles R. ; Kaur, Amanpreet ; Wright, Kathryn M. ; Lim, Tze Yin ; Jones, John ; Bryan, Glenn J. ; Blok, Vivian ; Hein, Ingo. / Mapping the H2 resistance effective against Globodera pallida pathotype Pa1 in tetraploid potato. In: Theoretical and Applied Genetics. 2019 ; Vol. 132, No. 4. pp. 1283-1294.
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abstract = "Key message: The nematode resistance gene H2 was mapped to the distal end of chromosome 5 in tetraploid potato. Abstract: The H2 resistance gene, introduced into cultivated potatoes from the wild diploid species Solanum multidissectum, confers a high level of resistance to the Pa1 pathotype of the potato cyst nematode Globodera pallida. A cross between tetraploid H2-containing breeding clone P55/7 and susceptible potato variety Picasso yielded an F1 population that segregated approximately 1:1 for the resistance phenotype, which is consistent with a single dominant gene in a simplex configuration. Using genome reduction methodologies RenSeq and GenSeq, the segregating F1 population enabled the genetic characterisation of the resistance through a bulked segregant analysis. A diagnostic RenSeq analysis of the parents confirmed that the resistance in P55/7 cannot be explained by previously characterised resistance genes. Only the variety Picasso contained functionally characterised disease resistance genes Rpi-R1, Rpi-R3a, Rpi-R3b variant, Gpa2 and Rx, which was independently confirmed through effector vacuum infiltration assays. RenSeq and GenSeq independently identified sequence polymorphisms linked to the H2 resistance on the top end of potato chromosome 5. Allele-specific KASP markers further defined the locus containing the H2 gene to a 4.7 Mb interval on the distal short arm of potato chromosome 5 and to positions that correspond to 1.4 MB and 6.1 MB in the potato reference genome.",
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Strachan, SM, Armstrong, MR, Kaur, A, Wright, KM, Lim, TY, Jones, J, Bryan, GJ, Blok, V & Hein, I 2019, 'Mapping the H2 resistance effective against Globodera pallida pathotype Pa1 in tetraploid potato', Theoretical and Applied Genetics, vol. 132, no. 4, pp. 1283-1294. https://doi.org/10.1007/s00122-019-03278-4

Mapping the H2 resistance effective against Globodera pallida pathotype Pa1 in tetraploid potato. / Strachan, Shona M .; Armstrong, Miles R.; Kaur, Amanpreet; Wright, Kathryn M.; Lim, Tze Yin ; Jones, John; Bryan, Glenn J.; Blok, Vivian; Hein, Ingo (Lead / Corresponding author).

In: Theoretical and Applied Genetics, Vol. 132, No. 4, 04.2019, p. 1283-1294.

Research output: Contribution to journalArticle

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T1 - Mapping the H2 resistance effective against Globodera pallida pathotype Pa1 in tetraploid potato

AU - Strachan, Shona M .

AU - Armstrong, Miles R.

AU - Kaur, Amanpreet

AU - Wright, Kathryn M.

AU - Lim, Tze Yin

AU - Jones, John

AU - Bryan, Glenn J.

AU - Blok, Vivian

AU - Hein, Ingo

N1 - Funding for this research was provided by: Biotechnology and Biological Sciences Research Council (BB/L008025/1, BB/K018299/1); Scottish Government (CRF/2009/SCRI/SOP 0929).

PY - 2019/4

Y1 - 2019/4

N2 - Key message: The nematode resistance gene H2 was mapped to the distal end of chromosome 5 in tetraploid potato. Abstract: The H2 resistance gene, introduced into cultivated potatoes from the wild diploid species Solanum multidissectum, confers a high level of resistance to the Pa1 pathotype of the potato cyst nematode Globodera pallida. A cross between tetraploid H2-containing breeding clone P55/7 and susceptible potato variety Picasso yielded an F1 population that segregated approximately 1:1 for the resistance phenotype, which is consistent with a single dominant gene in a simplex configuration. Using genome reduction methodologies RenSeq and GenSeq, the segregating F1 population enabled the genetic characterisation of the resistance through a bulked segregant analysis. A diagnostic RenSeq analysis of the parents confirmed that the resistance in P55/7 cannot be explained by previously characterised resistance genes. Only the variety Picasso contained functionally characterised disease resistance genes Rpi-R1, Rpi-R3a, Rpi-R3b variant, Gpa2 and Rx, which was independently confirmed through effector vacuum infiltration assays. RenSeq and GenSeq independently identified sequence polymorphisms linked to the H2 resistance on the top end of potato chromosome 5. Allele-specific KASP markers further defined the locus containing the H2 gene to a 4.7 Mb interval on the distal short arm of potato chromosome 5 and to positions that correspond to 1.4 MB and 6.1 MB in the potato reference genome.

AB - Key message: The nematode resistance gene H2 was mapped to the distal end of chromosome 5 in tetraploid potato. Abstract: The H2 resistance gene, introduced into cultivated potatoes from the wild diploid species Solanum multidissectum, confers a high level of resistance to the Pa1 pathotype of the potato cyst nematode Globodera pallida. A cross between tetraploid H2-containing breeding clone P55/7 and susceptible potato variety Picasso yielded an F1 population that segregated approximately 1:1 for the resistance phenotype, which is consistent with a single dominant gene in a simplex configuration. Using genome reduction methodologies RenSeq and GenSeq, the segregating F1 population enabled the genetic characterisation of the resistance through a bulked segregant analysis. A diagnostic RenSeq analysis of the parents confirmed that the resistance in P55/7 cannot be explained by previously characterised resistance genes. Only the variety Picasso contained functionally characterised disease resistance genes Rpi-R1, Rpi-R3a, Rpi-R3b variant, Gpa2 and Rx, which was independently confirmed through effector vacuum infiltration assays. RenSeq and GenSeq independently identified sequence polymorphisms linked to the H2 resistance on the top end of potato chromosome 5. Allele-specific KASP markers further defined the locus containing the H2 gene to a 4.7 Mb interval on the distal short arm of potato chromosome 5 and to positions that correspond to 1.4 MB and 6.1 MB in the potato reference genome.

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KW - H2

KW - tetraploid potato

KW - RenSeq

KW - dRenSeq

KW - GenSeq

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