TY - JOUR
T1 - Mass spectrometric analysis of the anaphase-promoting complex from yeast
T2 - Identification of a subunit related to cullins
AU - Zachariae, Wolfgang
AU - Shevchenko, Andrej
AU - Andrews, Paul D
AU - Ciosk, Rafael
AU - Galova, Marta
AU - Stark, Michael J R
AU - Mann, Matthias
AU - Nasmyth, Kim
PY - 1998
Y1 - 1998
N2 - Entry into anaphase and exit from mitosis depend on a ubiquitin-protein ligase complex called the anaphase-promoting complex (APC) or cyclosome. At least 12 different subunits were detected in the purified particle from budding yeast, including the previously identified proteins Apc1p, Cdc16p, Cdc23p, Cdc26p, and Cdc27p, Five additional subunits purified in low nanogram amounts were identified by tandem mass spectrometric sequencing, Apc2p, Apc5p, and the RING-finger protein Apc11p are conserved from yeast to humans, Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication.
AB - Entry into anaphase and exit from mitosis depend on a ubiquitin-protein ligase complex called the anaphase-promoting complex (APC) or cyclosome. At least 12 different subunits were detected in the purified particle from budding yeast, including the previously identified proteins Apc1p, Cdc16p, Cdc23p, Cdc26p, and Cdc27p, Five additional subunits purified in low nanogram amounts were identified by tandem mass spectrometric sequencing, Apc2p, Apc5p, and the RING-finger protein Apc11p are conserved from yeast to humans, Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication.
U2 - 10.1126/science.279.5354.1216
DO - 10.1126/science.279.5354.1216
M3 - Article
SN - 0036-8075
VL - 279
SP - 1216
EP - 1219
JO - Science
JF - Science
IS - 5354
ER -