Maternal dietary supplementation with saturated, but not monounsaturated or polyunsaturated fatty acids, leads to tissue-specific inhibition of offspring Na+,K+-ATPase

James A. Armitage, Sanjana Gupta, Caroline Wood, Runa I. Jensen, Anne-Maj Samuelsson, William Fuller, Michael J. Shattock, Lucilla Poston, Paul D. Taylor

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    Abstract

    In rats, a maternal diet rich in lard is associated with reduced Na+,K+-ATPase activity in adult offspring kidney. We have addressed the role of different fatty acids by evaluating Na+,K+-ATPase activity in offspring of dams fed diets rich in saturated (SFA), monounsaturated (MUFA) or polyunsaturated (PUFA) fatty acids. Female Sprague-Dawley rats were fed, during pregnancy and suckling, a control diet (4% w/w corn oil) or a fatty acid supplemented diet (24% w/w). Offspring were reared on chow (4% PUFA) and studied at 6 months. mRNA expression (real-time PCR) of Na+,K+-ATPase alpha subunit and protein expression of Na+,K+-ATPase subunits (Western blot) were assessed in kidney and brain. Na+,K+-ATPase activity was reduced in kidney (P < 0.05 versus all groups) and brain (P < 0.05 versus control and MUFA offspring) of the SFA group. Neither Na+,K+-ATPase alpha 1 subunit mRNA expression, nor protein expression of total alpha, alpha 1, alpha 2, alpha 3 or beta 1 subunits were significantly altered in kidney in any dietary group. In brains of SFA offspring alpha 1 mRNA expression (P < 0.05) was reduced compared with MUFA and PUFA offspring, but not controls. Also in brain, SFA offspring demonstrated reduced (P < 0.05) alpha 1 subunit protein and increased phosphorylation (P < 0.05) of the Na+,K+-ATPase modulating protein phospholemman at serine residue 63 (S63 PLM). Na+,K+-ATPase activity was similar to controls in heart and liver. In utero and neonatal exposure to a maternal diet rich in saturated fatty acids is associated with altered activity and expression of Na+,K+-ATPase in adulthood, but mechanisms appear tissue specific.

    Original languageEnglish
    Pages (from-to)5013-5022
    Number of pages10
    JournalJournal of Physiology
    Volume586
    Issue number20
    DOIs
    Publication statusPublished - 15 Oct 2008

    Keywords

    • NA-K-ATPASE
    • ALPHA-LINOLENIC ACID
    • BLOOD-PRESSURE LATER
    • METABOLIC SYNDROME
    • RAT
    • PREGNANCY
    • PHOSPHOLEMMAN
    • HYPERTENSION
    • DEFICIENCY
    • LIFE

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