Measurement of changes in blood oxygenation using Multispectral Optoacoustic Tomography (MSOT) allows assessment of tumor development

Michal R. Tomaszewski, Isabel Quiros-Gonzalez, James Joseph, Sarah E. Bohndiek

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


The ability to evaluate tumor oxygenation in the clinic could indicate prognosis and enable treatment monitoring, since oxygen deficient cancer cells are more resistant to chemotherapy and radiotherapy. MultiSpectral Optoacoustic Tomography (MSOT) is a hybrid technique combining the high contrast of optical imaging with the spatial resolution and penetration depth similar to ultrasound. We aim to demonstrate that MSOT can be used to monitor the development of tumor vasculature. To establish the relationship between MSOT derived imaging biomarkers and biological changes during tumor development, we performed MSOT on nude mice (n=10) bearing subcutaneous xenograft U87 glioblastoma tumors using a small animal optoacoustic tomography system. The mice were maintained under inhalation anesthesia during imaging and respired oxygen content was modified between 21% and 100%. The measurements from early (week 4) and late (week 7) stages of tumor development were compared. To further explore the functionality of the blood vessels, we examined the evolution of changes in the abundance of oxy-and deoxyhemoglobin in the tumors in response to a gas challenge. We found that the kinetics of the change in oxygen saturation (SO2) were significantly different between small tumors and the healthy blood vessels in nearby normal tissue (p=0.0054). Furthermore, we showed that there was a significant difference in the kinetics of the gas challenge between small and large tumors (p=0.0015). We also found that the tumor SO2 was significantly correlated (p=0.0057) with the tumor necrotic fraction as assessed by H&E staining in histology. In the future, this approach may be of use in the clinic as a method for tumor staging and assessment of treatment response.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2016
EditorsAlexander A. Oraevsky, Lihong V. Wang
PublisherSociety of Photo-optical Instrumentation Engineers
Number of pages8
ISBN (Electronic)9781628419429
Publication statusPublished - 15 Mar 2016
EventPhotons Plus Ultrasound: Imaging and Sensing 2016 - San Francisco, United States
Duration: 14 Feb 201617 Feb 2016

Publication series

NameProceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2016
Country/TerritoryUnited States
CitySan Francisco


  • MSOT
  • Optoacoustic
  • oxygenation
  • photoacoustic
  • tumor
  • vasculature

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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