Measurement of copper uptake in Saccharomyces cerevisiae using a Cu2+-selective electrode

L. De Rome, G. M. Gadd (Lead / Corresponding author)

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    49 Citations (Scopus)

    Abstract

    The uptake of copper by Saccharomyces cerevisiae was studied using a Cu2+-selective electrode and was found to be biphasic. The initial rapid metabolism-independent binding of Cu2+ to cell surfaces was followed by metabolism-dependent intracellular uptake. The later followed Michaelis-Menten kinetics with a Km of 1.13 ± 0.21 μM and Vmax of 2.22 ± 0.42 nmol Cu · min-1 · (mg dry wt)-1. Levels of intracellular K+ were critical for maximal Cu2+ uptake and it was found that the initial rate of K+ efflux was approximately twice that of the rate for Cu2+ influx.

    Original languageEnglish
    Pages (from-to)283-287
    Number of pages5
    JournalFEMS Microbiology Letters
    Volume43
    Issue number3
    DOIs
    Publication statusPublished - 1 Sep 1987

    Keywords

    • Copper uptake
    • Saccharomyces cerevisiae

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