Abstract
The uptake of copper by Saccharomyces cerevisiae was studied using a Cu2+-selective electrode and was found to be biphasic. The initial rapid metabolism-independent binding of Cu2+ to cell surfaces was followed by metabolism-dependent intracellular uptake. The later followed Michaelis-Menten kinetics with a Km of 1.13 ± 0.21 μM and Vmax of 2.22 ± 0.42 nmol Cu · min-1 · (mg dry wt)-1. Levels of intracellular K+ were critical for maximal Cu2+ uptake and it was found that the initial rate of K+ efflux was approximately twice that of the rate for Cu2+ influx.
Original language | English |
---|---|
Pages (from-to) | 283-287 |
Number of pages | 5 |
Journal | FEMS Microbiology Letters |
Volume | 43 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Sept 1987 |
Keywords
- Copper uptake
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics