The uptake of copper by Saccharomyces cerevisiae was studied using a Cu2+-selective electrode and was found to be biphasic. The initial rapid metabolism-independent binding of Cu2+ to cell surfaces was followed by metabolism-dependent intracellular uptake. The later followed Michaelis-Menten kinetics with a Km of 1.13 ± 0.21 μM and Vmax of 2.22 ± 0.42 nmol Cu · min-1 · (mg dry wt)-1. Levels of intracellular K+ were critical for maximal Cu2+ uptake and it was found that the initial rate of K+ efflux was approximately twice that of the rate for Cu2+ influx.
|Number of pages||5|
|Journal||FEMS Microbiology Letters|
|Publication status||Published - 1 Sep 1987|
- Copper uptake
- Saccharomyces cerevisiae