Mechanical stress induces profound remodelling of keratin filaments and cell junctions in epidermolysis bullosa simplex keratinocytes

David Russell, Paul D. Andrews, John James, E. Birgitte Lane

    Research output: Contribution to journalArticlepeer-review

    66 Citations (Scopus)

    Abstract

    The outer epidermal layer of the skin is an epithelium with remarkable protective barrier functions, which is subject to pronounced physical stress in its day-to-day function. A major candidate component for absorbing this stress is the K5/K14 keratin intermediate filament network. To investigate the part played by keratins in stress resilience, keratinocyte cell lines were subjected to mechanical stress. Repeated stretch and relaxation cycles over increasing time produced reproducible changes in the configuration of the keratin network. When wild-type cells were compared with cells carrying a keratin mutation associated with severe epidermolysis bullosa simplex-type skin fragility, the mutant keratin filaments were unable to withstand the mechanical stress and progressively fragmented yielding aggregates and novel ring structures. The cell junctions into which the keratin filaments are normally anchored also progressively disassembled, with all components tested of the cytoplasmic plaques becoming relocated away from the membrane and onto the keratin rings, while integral membrane receptors integrins and cadherins remained at the plasma membrane. The results suggest that maintenance of desmosomes and hemidesmosomes may require some tension, normally mediated by keratin attachments.
    Original languageEnglish
    Pages (from-to)5233-5243
    Number of pages11
    JournalJournal of Cell Science
    Volume117
    Issue number22
    DOIs
    Publication statusPublished - Oct 2004

    Keywords

    • Keratins
    • Desmosomes
    • Hemidesmosomes
    • Stress response
    • DM-EBS
    • Keratin mutations

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