Abstract
Original language | English |
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Article number | gkz613 |
Journal | Nucleic Acids Research |
Volume | 1 |
DOIs | |
Publication status | Published - 22 Jul 2019 |
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Keywords
- RNMT
- allosteric activation
- accelerated molecular dynamics
- conformational selection
- mRNA cap
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Mechanism of allosteric activation of human mRNA cap methyltransferase (RNMT) by RAM : Insights from accelerated molecular dynamics simulations. / Bueren-Calabuig, Juan (Lead / Corresponding author); Bage, Marcus; Cowling, Victoria; Pisliakov, Andrei (Lead / Corresponding author).
In: Nucleic Acids Research, Vol. 1, gkz613, 22.07.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mechanism of allosteric activation of human mRNA cap methyltransferase (RNMT) by RAM
T2 - Insights from accelerated molecular dynamics simulations
AU - Bueren-Calabuig, Juan
AU - Bage, Marcus
AU - Cowling, Victoria
AU - Pisliakov, Andrei
N1 - Research was funded by the Institutional Strategic Support Fund 204816 at the University of Dundee and Scottish Universities Physics Alliance (AVP), UK Medical Research Council Doctoral Training Programme (MB), and Medical Research Council MR/K024213/1 (VHC). Medical Research Council Senior Fellowship (MR/K024213/1), Royal Society Wolfson Research Merit Award (WRM\R1\180008), ERC Consolidator Grant 769080 TCAPS.
PY - 2019/7/22
Y1 - 2019/7/22
N2 - The RNA guanine-N7 methyltransferase (RNMT) in complex with RNMT-Activating Miniprotein (RAM) catalyses the formation of a N7-methylated guanosine cap structure on the 5’ end of nascent RNA polymerase II transcripts. The mRNA cap protects the primary transcript from exonucleases and recruits cap-binding complexes that mediate RNA processing, export and translation. By using microsecond standard and accelerated molecular dynamics simulations, we provide for the first time a detailed molecular mechanism of allosteric regulation of RNMT by RAM. We show that RAM selects the RNMT active site conformations that are optimal for binding of substrates (AdoMet and the cap), thus enhancing their affinity. Furthermore, our results strongly suggest the likely scenario in which the cap binding promotes the subsequent AdoMet binding, consistent with the previously suggested cooperative binding model. By employing the network community analyses, we revealed the underlying long-range allosteric networks and paths that are crucial for allosteric regulation by RAM. Our findings complement and explain previous experimental data on RNMT activity. Moreover, this study provides the most complete description of the cap and AdoMet binding poses and interactions within the enzyme’s active site. This information is critical for the drug discovery efforts that consider RNMT as a promising anti-cancer target.
AB - The RNA guanine-N7 methyltransferase (RNMT) in complex with RNMT-Activating Miniprotein (RAM) catalyses the formation of a N7-methylated guanosine cap structure on the 5’ end of nascent RNA polymerase II transcripts. The mRNA cap protects the primary transcript from exonucleases and recruits cap-binding complexes that mediate RNA processing, export and translation. By using microsecond standard and accelerated molecular dynamics simulations, we provide for the first time a detailed molecular mechanism of allosteric regulation of RNMT by RAM. We show that RAM selects the RNMT active site conformations that are optimal for binding of substrates (AdoMet and the cap), thus enhancing their affinity. Furthermore, our results strongly suggest the likely scenario in which the cap binding promotes the subsequent AdoMet binding, consistent with the previously suggested cooperative binding model. By employing the network community analyses, we revealed the underlying long-range allosteric networks and paths that are crucial for allosteric regulation by RAM. Our findings complement and explain previous experimental data on RNMT activity. Moreover, this study provides the most complete description of the cap and AdoMet binding poses and interactions within the enzyme’s active site. This information is critical for the drug discovery efforts that consider RNMT as a promising anti-cancer target.
KW - RNMT
KW - allosteric activation
KW - accelerated molecular dynamics
KW - conformational selection
KW - mRNA cap
U2 - 10.1093/nar/gkz613
DO - 10.1093/nar/gkz613
M3 - Article
C2 - 31329932
VL - 1
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
M1 - gkz613
ER -