Membrane disruption by optically controlled microbubble cavitation

Paul Prentice, Alfred Cuschieri, Kishan Dholakia, Mark Prausnitz, Paul Campbell

    Research output: Contribution to journalArticle

    393 Citations (Scopus)

    Abstract

    In fluids, pressure-driven cavitation bubbles have a nonlinear response that can lead to extremely high core-energy densities during the collapse phase—a process underpinning phenomena such as sonoluminescence1 and plasma formation2. If cavitation occurs near a rigid surface, the bubbles tend to collapse asymmetrically, often forming fast-moving liquid jets that may create localized surface damage3. As encapsulated microbubbles are commonly used to improve echo generation in diagnostic ultrasound imaging, it is possible that such cavitation could also lead to jet-induced tissue damage. Certainly ultrasonic irradiation (insonation) of cells in the presence of microbubbles can lead to enhanced membrane permeabilization and molecular uptake (sonoporation)4, 5, 6, 7, but, although the mechanism during low-intensity insonation is clear8, experimental corroboration for higher pressure regimes has remained elusive. Here we show direct observational evidence that illuminates the energetic micrometre-scale interactions between individual cells and violently cavitating shelled microbubbles. Our data suggest that sonoporation at higher intensities may arise through a synergistic interplay involving several distinct processes.
    Original languageEnglish
    Pages (from-to)107-110
    Number of pages4
    JournalNature Physics
    Volume1
    Issue number2
    DOIs
    Publication statusPublished - Nov 2005

    Fingerprint

    cavitation flow
    membranes
    bubbles
    fluid pressure
    cells
    micrometers
    echoes
    flux density
    ultrasonics
    damage
    irradiation
    liquids
    interactions

    Keywords

    • Biological physics
    • Techniques and instrumentation
    • Fluid dynamics
    • Microbubbles

    Cite this

    Prentice, Paul ; Cuschieri, Alfred ; Dholakia, Kishan ; Prausnitz, Mark ; Campbell, Paul. / Membrane disruption by optically controlled microbubble cavitation. In: Nature Physics. 2005 ; Vol. 1, No. 2. pp. 107-110.
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    abstract = "In fluids, pressure-driven cavitation bubbles have a nonlinear response that can lead to extremely high core-energy densities during the collapse phase—a process underpinning phenomena such as sonoluminescence1 and plasma formation2. If cavitation occurs near a rigid surface, the bubbles tend to collapse asymmetrically, often forming fast-moving liquid jets that may create localized surface damage3. As encapsulated microbubbles are commonly used to improve echo generation in diagnostic ultrasound imaging, it is possible that such cavitation could also lead to jet-induced tissue damage. Certainly ultrasonic irradiation (insonation) of cells in the presence of microbubbles can lead to enhanced membrane permeabilization and molecular uptake (sonoporation)4, 5, 6, 7, but, although the mechanism during low-intensity insonation is clear8, experimental corroboration for higher pressure regimes has remained elusive. Here we show direct observational evidence that illuminates the energetic micrometre-scale interactions between individual cells and violently cavitating shelled microbubbles. Our data suggest that sonoporation at higher intensities may arise through a synergistic interplay involving several distinct processes.",
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    Membrane disruption by optically controlled microbubble cavitation. / Prentice, Paul; Cuschieri, Alfred; Dholakia, Kishan; Prausnitz, Mark; Campbell, Paul.

    In: Nature Physics, Vol. 1, No. 2, 11.2005, p. 107-110.

    Research output: Contribution to journalArticle

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    T1 - Membrane disruption by optically controlled microbubble cavitation

    AU - Prentice, Paul

    AU - Cuschieri, Alfred

    AU - Dholakia, Kishan

    AU - Prausnitz, Mark

    AU - Campbell, Paul

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    N2 - In fluids, pressure-driven cavitation bubbles have a nonlinear response that can lead to extremely high core-energy densities during the collapse phase—a process underpinning phenomena such as sonoluminescence1 and plasma formation2. If cavitation occurs near a rigid surface, the bubbles tend to collapse asymmetrically, often forming fast-moving liquid jets that may create localized surface damage3. As encapsulated microbubbles are commonly used to improve echo generation in diagnostic ultrasound imaging, it is possible that such cavitation could also lead to jet-induced tissue damage. Certainly ultrasonic irradiation (insonation) of cells in the presence of microbubbles can lead to enhanced membrane permeabilization and molecular uptake (sonoporation)4, 5, 6, 7, but, although the mechanism during low-intensity insonation is clear8, experimental corroboration for higher pressure regimes has remained elusive. Here we show direct observational evidence that illuminates the energetic micrometre-scale interactions between individual cells and violently cavitating shelled microbubbles. Our data suggest that sonoporation at higher intensities may arise through a synergistic interplay involving several distinct processes.

    AB - In fluids, pressure-driven cavitation bubbles have a nonlinear response that can lead to extremely high core-energy densities during the collapse phase—a process underpinning phenomena such as sonoluminescence1 and plasma formation2. If cavitation occurs near a rigid surface, the bubbles tend to collapse asymmetrically, often forming fast-moving liquid jets that may create localized surface damage3. As encapsulated microbubbles are commonly used to improve echo generation in diagnostic ultrasound imaging, it is possible that such cavitation could also lead to jet-induced tissue damage. Certainly ultrasonic irradiation (insonation) of cells in the presence of microbubbles can lead to enhanced membrane permeabilization and molecular uptake (sonoporation)4, 5, 6, 7, but, although the mechanism during low-intensity insonation is clear8, experimental corroboration for higher pressure regimes has remained elusive. Here we show direct observational evidence that illuminates the energetic micrometre-scale interactions between individual cells and violently cavitating shelled microbubbles. Our data suggest that sonoporation at higher intensities may arise through a synergistic interplay involving several distinct processes.

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