Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus

Franziska Jager; Martin Zoltner; Holger Kneuper; William N. Hunter; Tracy Palmer

doi:10.1002/1873-3468.12065

Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus

Franziska Jager, Martin Zoltner, Holger Kneuper, William N. Hunter, Tracy Palmer (Lead / Corresponding author)

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Abstract

The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.

Original language	English
Pages (from-to)	349-357
Number of pages	9
Journal	FEBS Letters
Volume	590
Issue number	3
Early online date	3 Feb 2016
DOIs	https://doi.org/10.1002/1873-3468.12065
Publication status	Published - 12 Feb 2016

Keywords

membrane protein complex
protein secretion
Staphylococcusaureus
Type VII secretion

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10.1002/1873-3468.12065Licence: CC BY

Publisher final version
© 2016 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited
Final published version, 547 KBLicence: CC BY

Characterisation of the Ess Protein Secretion System of Staphylococcus Aureus a Key Virulence Factor
Hunter, B. (Investigator) & Palmer, T. (Investigator)
Medical Research Council
1/04/15 → 31/05/18
Project: Research

Cite this

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abstract = "The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.",

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AU - Zoltner, Martin

AU - Kneuper, Holger

AU - Hunter, William N.

AU - Palmer, Tracy

PY - 2016/2/12

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N2 - The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.

AB - The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.

KW - membrane protein complex

KW - protein secretion

KW - Staphylococcusaureus

KW - Type VII secretion

U2 - 10.1002/1873-3468.12065

DO - 10.1002/1873-3468.12065

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VL - 590

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EP - 357

JO - FEBS Letters

JF - FEBS Letters

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ER -

Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus

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Characterisation of the Ess Protein Secretion System of Staphylococcus Aureus a Key Virulence Factor

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