Abstract
Euglena spp. are phototrophic flagellates with considerable ecological presence and impact. Euglena gracilis harbours secondary green plastids, but an incompletely characterised proteome precludes accurate understanding of both plastid function and evolutionary history. Using subcellular fractionation, an improved sequence database and MS we determined the composition, evolutionary relationships and hence predicted functions of the E. gracilis plastid proteome. We confidently identified 1345 distinct plastid protein groups and found that at least 100 proteins represent horizontal acquisitions from organisms other than green algae or prokaryotes. Metabolic reconstruction confirmed previously studied/predicted enzymes/pathways and provided evidence for multiple unusual features, including uncoupling of carotenoid and phytol metabolism, a limited role in amino acid metabolism, and dual sets of the SUF pathway for FeS cluster assembly, one of which was acquired by lateral gene transfer from Chlamydiae. Plastid paralogues of trafficking-associated proteins potentially mediating fusion of transport vesicles with the outermost plastid membrane were identified, together with derlin-related proteins, potential translocases across the middle membrane, and an extremely simplified TIC complex. The Euglena plastid, as the product of many genomes, combines novel and conserved features of metabolism and transport.
| Original language | English |
|---|---|
| Number of pages | 15 |
| Journal | New Phytologist |
| Early online date | 3 Oct 2019 |
| DOIs | |
| Publication status | E-pub ahead of print - 3 Oct 2019 |
Fingerprint
Keywords
- Euglena gracilis
- SUF pathway
- lateral gene transfer
- metabolic reconstruction
- plastid
- protein import
- proteome
- shopping bag hypothesis
Cite this
}
Metabolic quirks and the colourful history of the Euglena gracilis secondary plastid. / Novák Vanclová, Anna M. G.; Zoltner, Martin; Kelly, Steven; Soukal, Petr; Záhonová, Kristína; Füssy, Zoltán; Ebenezer, ThankGod E.; Lacová Dobáková, Eva; Eliáš, Marek; Lukeš, Julius; Field, Mark C.; Hampl, Vladimír.
In: New Phytologist, 03.10.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - Metabolic quirks and the colourful history of the Euglena gracilis secondary plastid
AU - Novák Vanclová, Anna M. G.
AU - Zoltner, Martin
AU - Kelly, Steven
AU - Soukal, Petr
AU - Záhonová, Kristína
AU - Füssy, Zoltán
AU - Ebenezer, ThankGod E.
AU - Lacová Dobáková, Eva
AU - Eliáš, Marek
AU - Lukeš, Julius
AU - Field, Mark C.
AU - Hampl, Vladimír
N1 - © 2019 The Authors New Phytologist © 2019 New Phytologist Trust.
PY - 2019/10/3
Y1 - 2019/10/3
N2 - Euglena spp. are phototrophic flagellates with considerable ecological presence and impact. Euglena gracilis harbours secondary green plastids, but an incompletely characterised proteome precludes accurate understanding of both plastid function and evolutionary history. Using subcellular fractionation, an improved sequence database and MS we determined the composition, evolutionary relationships and hence predicted functions of the E. gracilis plastid proteome. We confidently identified 1345 distinct plastid protein groups and found that at least 100 proteins represent horizontal acquisitions from organisms other than green algae or prokaryotes. Metabolic reconstruction confirmed previously studied/predicted enzymes/pathways and provided evidence for multiple unusual features, including uncoupling of carotenoid and phytol metabolism, a limited role in amino acid metabolism, and dual sets of the SUF pathway for FeS cluster assembly, one of which was acquired by lateral gene transfer from Chlamydiae. Plastid paralogues of trafficking-associated proteins potentially mediating fusion of transport vesicles with the outermost plastid membrane were identified, together with derlin-related proteins, potential translocases across the middle membrane, and an extremely simplified TIC complex. The Euglena plastid, as the product of many genomes, combines novel and conserved features of metabolism and transport.
AB - Euglena spp. are phototrophic flagellates with considerable ecological presence and impact. Euglena gracilis harbours secondary green plastids, but an incompletely characterised proteome precludes accurate understanding of both plastid function and evolutionary history. Using subcellular fractionation, an improved sequence database and MS we determined the composition, evolutionary relationships and hence predicted functions of the E. gracilis plastid proteome. We confidently identified 1345 distinct plastid protein groups and found that at least 100 proteins represent horizontal acquisitions from organisms other than green algae or prokaryotes. Metabolic reconstruction confirmed previously studied/predicted enzymes/pathways and provided evidence for multiple unusual features, including uncoupling of carotenoid and phytol metabolism, a limited role in amino acid metabolism, and dual sets of the SUF pathway for FeS cluster assembly, one of which was acquired by lateral gene transfer from Chlamydiae. Plastid paralogues of trafficking-associated proteins potentially mediating fusion of transport vesicles with the outermost plastid membrane were identified, together with derlin-related proteins, potential translocases across the middle membrane, and an extremely simplified TIC complex. The Euglena plastid, as the product of many genomes, combines novel and conserved features of metabolism and transport.
KW - Euglena gracilis
KW - SUF pathway
KW - lateral gene transfer
KW - metabolic reconstruction
KW - plastid
KW - protein import
KW - proteome
KW - shopping bag hypothesis
UR - http://www.scopus.com/inward/record.url?scp=85074777027&partnerID=8YFLogxK
U2 - 10.1111/nph.16237
DO - 10.1111/nph.16237
M3 - Article
C2 - 31580486
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
ER -