Metabolic quirks and the colourful history of the Euglena gracilis secondary plastid

Anna M. G. Novák Vanclová, Martin Zoltner, Steven Kelly, Petr Soukal, Kristína Záhonová, Zoltán Füssy, ThankGod E. Ebenezer, Eva Lacová Dobáková, Marek Eliáš, Julius Lukeš, Mark C. Field, Vladimír Hampl

Research output: Contribution to journalArticle

Abstract

Euglena spp. are phototrophic flagellates with considerable ecological presence and impact. Euglena gracilis harbours secondary green plastids, but an incompletely characterised proteome precludes accurate understanding of both plastid function and evolutionary history. Using subcellular fractionation, an improved sequence database and MS we determined the composition, evolutionary relationships and hence predicted functions of the E. gracilis plastid proteome. We confidently identified 1345 distinct plastid protein groups and found that at least 100 proteins represent horizontal acquisitions from organisms other than green algae or prokaryotes. Metabolic reconstruction confirmed previously studied/predicted enzymes/pathways and provided evidence for multiple unusual features, including uncoupling of carotenoid and phytol metabolism, a limited role in amino acid metabolism, and dual sets of the SUF pathway for FeS cluster assembly, one of which was acquired by lateral gene transfer from Chlamydiae. Plastid paralogues of trafficking-associated proteins potentially mediating fusion of transport vesicles with the outermost plastid membrane were identified, together with derlin-related proteins, potential translocases across the middle membrane, and an extremely simplified TIC complex. The Euglena plastid, as the product of many genomes, combines novel and conserved features of metabolism and transport.

Original languageEnglish
Number of pages15
JournalNew Phytologist
Early online date3 Oct 2019
DOIs
Publication statusE-pub ahead of print - 3 Oct 2019

Fingerprint

Euglena gracilis
Plastids
plastids
History
history
Euglena
Proteome
proteome
Phytol
Chloroplast Proteins
proteins
Horizontal Gene Transfer
Transport Vesicles
Chlorophyta
Membranes
Chlamydia
Protein Transport
Carotenoids
metabolism
amino acid metabolism

Keywords

  • Euglena gracilis
  • SUF pathway
  • lateral gene transfer
  • metabolic reconstruction
  • plastid
  • protein import
  • proteome
  • shopping bag hypothesis

Cite this

Novák Vanclová, A. M. G., Zoltner, M., Kelly, S., Soukal, P., Záhonová, K., Füssy, Z., ... Hampl, V. (2019). Metabolic quirks and the colourful history of the Euglena gracilis secondary plastid. New Phytologist. https://doi.org/10.1111/nph.16237
Novák Vanclová, Anna M. G. ; Zoltner, Martin ; Kelly, Steven ; Soukal, Petr ; Záhonová, Kristína ; Füssy, Zoltán ; Ebenezer, ThankGod E. ; Lacová Dobáková, Eva ; Eliáš, Marek ; Lukeš, Julius ; Field, Mark C. ; Hampl, Vladimír. / Metabolic quirks and the colourful history of the Euglena gracilis secondary plastid. In: New Phytologist. 2019.
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abstract = "Euglena spp. are phototrophic flagellates with considerable ecological presence and impact. Euglena gracilis harbours secondary green plastids, but an incompletely characterised proteome precludes accurate understanding of both plastid function and evolutionary history. Using subcellular fractionation, an improved sequence database and MS we determined the composition, evolutionary relationships and hence predicted functions of the E. gracilis plastid proteome. We confidently identified 1345 distinct plastid protein groups and found that at least 100 proteins represent horizontal acquisitions from organisms other than green algae or prokaryotes. Metabolic reconstruction confirmed previously studied/predicted enzymes/pathways and provided evidence for multiple unusual features, including uncoupling of carotenoid and phytol metabolism, a limited role in amino acid metabolism, and dual sets of the SUF pathway for FeS cluster assembly, one of which was acquired by lateral gene transfer from Chlamydiae. Plastid paralogues of trafficking-associated proteins potentially mediating fusion of transport vesicles with the outermost plastid membrane were identified, together with derlin-related proteins, potential translocases across the middle membrane, and an extremely simplified TIC complex. The Euglena plastid, as the product of many genomes, combines novel and conserved features of metabolism and transport.",
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Novák Vanclová, AMG, Zoltner, M, Kelly, S, Soukal, P, Záhonová, K, Füssy, Z, Ebenezer, TE, Lacová Dobáková, E, Eliáš, M, Lukeš, J, Field, MC & Hampl, V 2019, 'Metabolic quirks and the colourful history of the Euglena gracilis secondary plastid', New Phytologist. https://doi.org/10.1111/nph.16237

Metabolic quirks and the colourful history of the Euglena gracilis secondary plastid. / Novák Vanclová, Anna M. G.; Zoltner, Martin; Kelly, Steven; Soukal, Petr; Záhonová, Kristína; Füssy, Zoltán; Ebenezer, ThankGod E.; Lacová Dobáková, Eva; Eliáš, Marek; Lukeš, Julius; Field, Mark C.; Hampl, Vladimír.

In: New Phytologist, 03.10.2019.

Research output: Contribution to journalArticle

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AU - Novák Vanclová, Anna M. G.

AU - Zoltner, Martin

AU - Kelly, Steven

AU - Soukal, Petr

AU - Záhonová, Kristína

AU - Füssy, Zoltán

AU - Ebenezer, ThankGod E.

AU - Lacová Dobáková, Eva

AU - Eliáš, Marek

AU - Lukeš, Julius

AU - Field, Mark C.

AU - Hampl, Vladimír

N1 - © 2019 The Authors New Phytologist © 2019 New Phytologist Trust.

PY - 2019/10/3

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KW - lateral gene transfer

KW - metabolic reconstruction

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KW - protein import

KW - proteome

KW - shopping bag hypothesis

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