Metabolic requirements for phorbol ester-induced cytoskeletal changes in renal epithelium

Maeve A. Rahilly, Stewart Fleming

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Alteration of the cytoskeleton and cell-substratum adhesion are important in the progression of renal carcinoma. We have previously shown that treatment of normal human renal epithelium with phorbol esters mimics the changes seen in renal carcinoma cells. In this study we have demonstrated that the phorbol ester-induced cytoskeletal reorganization is inhibited in the presence of deoxyglucose but not by cycloheximide. We have also shown that treatment of cells with cytochalasin B results in the formation of immature stress fibres restricted to the cell-substratum contact regions. These results suggest that the actin filaments elongate from the focal contacts and that structural rearrangement caused by phorbol esters is an energy-dependent phenomenon but is independent of de novo protein synthesis.
    Original languageEnglish
    Pages (from-to)439-43
    Number of pages5
    JournalJournal of Pathology
    Volume169
    Issue number4
    DOIs
    Publication statusPublished - 1993

    Fingerprint

    Phorbol Esters
    Epithelium
    Kidney
    Stress Fibers
    Cytochalasin B
    Focal Adhesions
    Deoxyglucose
    Cycloheximide
    Cytoskeleton
    Actin Cytoskeleton
    Renal Cell Carcinoma
    Cell Adhesion
    Carcinoma
    Proteins

    Cite this

    @article{c747430aee01471d8a19320957bde974,
    title = "Metabolic requirements for phorbol ester-induced cytoskeletal changes in renal epithelium",
    abstract = "Alteration of the cytoskeleton and cell-substratum adhesion are important in the progression of renal carcinoma. We have previously shown that treatment of normal human renal epithelium with phorbol esters mimics the changes seen in renal carcinoma cells. In this study we have demonstrated that the phorbol ester-induced cytoskeletal reorganization is inhibited in the presence of deoxyglucose but not by cycloheximide. We have also shown that treatment of cells with cytochalasin B results in the formation of immature stress fibres restricted to the cell-substratum contact regions. These results suggest that the actin filaments elongate from the focal contacts and that structural rearrangement caused by phorbol esters is an energy-dependent phenomenon but is independent of de novo protein synthesis.",
    author = "Rahilly, {Maeve A.} and Stewart Fleming",
    year = "1993",
    doi = "10.1002/path.1711690409",
    language = "English",
    volume = "169",
    pages = "439--43",
    journal = "Journal of Pathology",
    issn = "0022-3417",
    publisher = "Wiley",
    number = "4",

    }

    Metabolic requirements for phorbol ester-induced cytoskeletal changes in renal epithelium. / Rahilly, Maeve A.; Fleming, Stewart.

    In: Journal of Pathology, Vol. 169, No. 4, 1993, p. 439-43.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Metabolic requirements for phorbol ester-induced cytoskeletal changes in renal epithelium

    AU - Rahilly, Maeve A.

    AU - Fleming, Stewart

    PY - 1993

    Y1 - 1993

    N2 - Alteration of the cytoskeleton and cell-substratum adhesion are important in the progression of renal carcinoma. We have previously shown that treatment of normal human renal epithelium with phorbol esters mimics the changes seen in renal carcinoma cells. In this study we have demonstrated that the phorbol ester-induced cytoskeletal reorganization is inhibited in the presence of deoxyglucose but not by cycloheximide. We have also shown that treatment of cells with cytochalasin B results in the formation of immature stress fibres restricted to the cell-substratum contact regions. These results suggest that the actin filaments elongate from the focal contacts and that structural rearrangement caused by phorbol esters is an energy-dependent phenomenon but is independent of de novo protein synthesis.

    AB - Alteration of the cytoskeleton and cell-substratum adhesion are important in the progression of renal carcinoma. We have previously shown that treatment of normal human renal epithelium with phorbol esters mimics the changes seen in renal carcinoma cells. In this study we have demonstrated that the phorbol ester-induced cytoskeletal reorganization is inhibited in the presence of deoxyglucose but not by cycloheximide. We have also shown that treatment of cells with cytochalasin B results in the formation of immature stress fibres restricted to the cell-substratum contact regions. These results suggest that the actin filaments elongate from the focal contacts and that structural rearrangement caused by phorbol esters is an energy-dependent phenomenon but is independent of de novo protein synthesis.

    U2 - 10.1002/path.1711690409

    DO - 10.1002/path.1711690409

    M3 - Article

    VL - 169

    SP - 439

    EP - 443

    JO - Journal of Pathology

    JF - Journal of Pathology

    SN - 0022-3417

    IS - 4

    ER -