Metabolism of carbon tetrachloride in hepatic microsomes and reconstituted monooxygenase systems and its relationship to lipid peroxidation

C. R. Wolf, W. G. Harrelson, W. M. Nastainczyk, R. M. Philpot, B. Kalyanaraman, R. P. Mason

    Research output: Contribution to journalArticlepeer-review

    98 Citations (Scopus)

    Abstract

    The metabolism of carbon tetrachloride has been investigated in liver microsomes and in reconstituted cytochrome P-450-dependent monooxygenase systems. In both cases cytochrome P-450 appears to be the sole site of carbon tetrachloride reduction. In aerobic incubations of microsomal preparations from phenobarbital-treated rats or rabbits, carbon tetrachloride induces large increases in NADPH oxidation and oxygen uptake. This cofactor utilization is not directly related to monooxygenase-mediated metabolism. Under aerobic conditions carbon tetrachloride induces lipid peroxidation, a reaction which is not inhibited by carbon monoxide. Under anaerobic conditions, carbon monoxide is a potent inhibitor of the cytochrome P-450-mediated reduction of carbon tetrachloride. These facts are inconsistent with a role for carbon tetrachloride metabolism in the potentiation of lipid peroxidation.

    Original languageEnglish
    Pages (from-to)553-558
    Number of pages6
    JournalMolecular Pharmacology
    Volume18
    Issue number3
    Publication statusPublished - 1 Dec 1980

    ASJC Scopus subject areas

    • General Medicine
    • Molecular Medicine
    • Pharmacology

    Fingerprint

    Dive into the research topics of 'Metabolism of carbon tetrachloride in hepatic microsomes and reconstituted monooxygenase systems and its relationship to lipid peroxidation'. Together they form a unique fingerprint.

    Cite this