Metabolism of carbon tetrachloride in hepatic microsomes and reconstituted monooxygenase systems and its relationship to lipid peroxidation

C. R. Wolf, W. G. Harrelson, W. M. Nastainczyk, R. M. Philpot, B. Kalyanaraman, R. P. Mason

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Abstract

The metabolism of carbon tetrachloride has been investigated in liver microsomes and in reconstituted cytochrome P-450-dependent monooxygenase systems. In both cases cytochrome P-450 appears to be the sole site of carbon tetrachloride reduction. In aerobic incubations of microsomal preparations from phenobarbital-treated rats or rabbits, carbon tetrachloride induces large increases in NADPH oxidation and oxygen uptake. This cofactor utilization is not directly related to monooxygenase-mediated metabolism. Under aerobic conditions carbon tetrachloride induces lipid peroxidation, a reaction which is not inhibited by carbon monoxide. Under anaerobic conditions, carbon monoxide is a potent inhibitor of the cytochrome P-450-mediated reduction of carbon tetrachloride. These facts are inconsistent with a role for carbon tetrachloride metabolism in the potentiation of lipid peroxidation.

Original languageEnglish
Pages (from-to)553-558
Number of pages6
JournalMolecular Pharmacology
Volume18
Issue number3
Publication statusPublished - 1 Dec 1980

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    Wolf, C. R., Harrelson, W. G., Nastainczyk, W. M., Philpot, R. M., Kalyanaraman, B., & Mason, R. P. (1980). Metabolism of carbon tetrachloride in hepatic microsomes and reconstituted monooxygenase systems and its relationship to lipid peroxidation. Molecular Pharmacology, 18(3), 553-558.