TY - JOUR
T1 - Metagenomic insights into the abundance and composition of resistance genes in aquatic environments
T2 - Influence of stratification and geography
AU - Yang, Yuyi
AU - Li, Zan
AU - Song, Wenjuan
AU - Du, Linna
AU - Ye, Chen
AU - Zhao, Bo
AU - Liu, Wenzhi
AU - Deng, Danli
AU - Pan, Yongtai
AU - Lin, Hui
AU - Cao, Xinhua
N1 - This work was supported by the National Natural Science Foundation of China [grant number 41673127], the Youth Innovation Promotion Association of the Chinese Academy of Sciences [grant numbers 2015282 and 2017388], the Wenzhou Science and Technology Major Project [ZS2017001], and Basic Public Welfare Research Projects in Zhejiang Province [LGF18E090007].
PY - 2019/6
Y1 - 2019/6
N2 - A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative abundances of ARGs and MRGs in lake samples were in the ranges ND (not detected)–1.34 × 10
0 and 1.22 × 10
−3 –1.98 × 10
−1 copies per 16S rRNA, which were higher than those in seawater samples. Among ARGs, multidrug resistance genes and bacitracin resistance genes had high relative abundances in both lake and sea water samples. Multi-metal resistance genes, mercury resistance genes and copper resistance genes had the greatest relative abundance for MRGs. No significant difference was found between epilimnion and hypolimnion in abundance or the Shannon diversity index for ARGs and MRGs. Principal coordinates analysis and permutational multivariate analysis of variance (PERMANOVA) test showed that stratification and geography had significant influence on the composition of ARGs and MRGs in lakes (p < 0.05, PERMANOVA). Coastal seawater samples had significantly greater relative abundance and a higher Shannon index for both ARGs and MRGs than deep ocean and Antarctic seawater samples (p < 0.05, Kruskal-Wallis one-way ANOVA), suggesting that human activity may exert more selective pressure on ARGs and MRGs in coastal areas than those in deep ocean and Antarctic seawater.
AB - A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative abundances of ARGs and MRGs in lake samples were in the ranges ND (not detected)–1.34 × 10
0 and 1.22 × 10
−3 –1.98 × 10
−1 copies per 16S rRNA, which were higher than those in seawater samples. Among ARGs, multidrug resistance genes and bacitracin resistance genes had high relative abundances in both lake and sea water samples. Multi-metal resistance genes, mercury resistance genes and copper resistance genes had the greatest relative abundance for MRGs. No significant difference was found between epilimnion and hypolimnion in abundance or the Shannon diversity index for ARGs and MRGs. Principal coordinates analysis and permutational multivariate analysis of variance (PERMANOVA) test showed that stratification and geography had significant influence on the composition of ARGs and MRGs in lakes (p < 0.05, PERMANOVA). Coastal seawater samples had significantly greater relative abundance and a higher Shannon index for both ARGs and MRGs than deep ocean and Antarctic seawater samples (p < 0.05, Kruskal-Wallis one-way ANOVA), suggesting that human activity may exert more selective pressure on ARGs and MRGs in coastal areas than those in deep ocean and Antarctic seawater.
KW - Bacitracin resistance genes
KW - Mercury resistance genes
KW - Metagenomic analysis
KW - Principal coordinates analysis
KW - Stratification
UR - http://www.scopus.com/inward/record.url?scp=85063758269&partnerID=8YFLogxK
U2 - 10.1016/j.envint.2019.03.062
DO - 10.1016/j.envint.2019.03.062
M3 - Article
C2 - 30954723
SN - 0160-4120
VL - 127
SP - 371
EP - 380
JO - Environment International
JF - Environment International
ER -