Methods for studying the DNA damage response in the Caenorhabdatis elegans germ line

Ashley L. Craig, Sandra C. Moser, Aymeric P. Bailly, Anton Gartner

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    56 Citations (Scopus)

    Abstract

    In response to genotoxic insults, cells activate DNA damage response pathways that either stimulate transient cell cycle arrest and DNA repair or induce apoptosis. The Caenorhabditis elegans germ line is now well established as a model system to study these processes in a genetically tractable, multicellular organism. Upon treatment with genotoxic agents, premeiotic C. elegans germ cells transiently halt cell cycle progression, whereas meiotic prophase germ cells in the late-pachytene stage undergo apoptosis. Further, accumulation of unrepaired meiotic recombination intermediates can also lead to apoptosis of affected pachytene cells. DNA damage-induced cell death requires key components of the evolutionarily conserved apoptotic machinery. Moreover, both cell cycle arrest and pachytene apoptosis responses depend on conserved DNA damage checkpoint proteins. Genetics- and genomics-based approaches that have demonstrated roles for conserved checkpoint proteins have also begun to uncover novel components of these response pathways. In this chapter, we briefly review the C. elegans DNA damage response field, discuss in detail methods currently used to assay DNA damage responses in C. elegans, and describe the development of new experimental tools that will facilitate a more comprehensive understanding of the DNA damage response.
    Original languageEnglish
    Title of host publicationCaenorhabditis elegans
    Subtitle of host publicationcell biology and physiology
    EditorsJoel H. Rothman, Andrew Singson
    PublisherAcademic Press
    Pages321-352
    Number of pages32
    ISBN (Print)9780123946201
    DOIs
    Publication statusPublished - 2012

    Publication series

    NameMethods in Cell Biology
    Volume107
    ISSN (Print)0091-679X

    Keywords

    • Genotoxic
    • Germ cell
    • Loss-of-function
    • Progeny
    • Recombinase
    • Recombination

    Fingerprint Dive into the research topics of 'Methods for studying the DNA damage response in the Caenorhabdatis elegans germ line'. Together they form a unique fingerprint.

    Cite this