Methods to investigate the effects of chronic ethanol on adipocytes

Becky M. Sebastian; Li Kang; Xiaocong Chen; Laura E. Nagy

doi:10.1007/978-1-59745-242-7_23

Methods to investigate the effects of chronic ethanol on adipocytes

Becky M. Sebastian, Li Kang, Xiaocong Chen, Laura E. Nagy

Systems Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Chronic ethanol consumption dysregulates glucose and lipid homeostasis, is associated with insulin resistance, and alters serum levels of adipokines including adiponectin and tumor necrosis factor-alpha. However, the mechanisms involved in these chronic ethanol-induced pathologies are not fully understood. Adipose tissue has been implicated as an important contributor to chronic ethanol-induced disease states and, therefore, the effects of chronic ethanol feeding in rats on adipocytes has been investigated. Three major functions of the adipocyte include glucose transport, adipokine secretion, and triglyceride breakdown via lipolysis. Included in this chapter are protocols for studying the effect of chronic ethanol feeding on these adipocyte functions.

Original language	English
Pages (from-to)	357-66
Number of pages	10
Journal	Methods in Molecular Biology (Clifton, N.J.)
Volume	447
DOIs	https://doi.org/10.1007/978-1-59745-242-7_23
Publication status	Published - 2008

Keywords

Adipocytes/drug effects
Adiponectin/metabolism
Adrenergic beta-Agonists/pharmacology
Animals
Biological Assay/methods
Cell Culture Techniques
Cell Separation
Cells, Cultured
Ethanol/toxicity
Glucose/metabolism
Insulin/metabolism
Isoproterenol/pharmacology
Lipolysis/drug effects
Rats

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10.1007/978-1-59745-242-7_23

Cite this

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title = "Methods to investigate the effects of chronic ethanol on adipocytes",

abstract = "Chronic ethanol consumption dysregulates glucose and lipid homeostasis, is associated with insulin resistance, and alters serum levels of adipokines including adiponectin and tumor necrosis factor-alpha. However, the mechanisms involved in these chronic ethanol-induced pathologies are not fully understood. Adipose tissue has been implicated as an important contributor to chronic ethanol-induced disease states and, therefore, the effects of chronic ethanol feeding in rats on adipocytes has been investigated. Three major functions of the adipocyte include glucose transport, adipokine secretion, and triglyceride breakdown via lipolysis. Included in this chapter are protocols for studying the effect of chronic ethanol feeding on these adipocyte functions.",

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author = "Sebastian, {Becky M.} and Li Kang and Xiaocong Chen and Nagy, {Laura E.}",

year = "2008",

doi = "10.1007/978-1-59745-242-7_23",

language = "English",

volume = "447",

pages = "357--66",

journal = "Methods in Molecular Biology (Clifton, N.J.)",

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T1 - Methods to investigate the effects of chronic ethanol on adipocytes

AU - Sebastian, Becky M.

AU - Kang, Li

AU - Chen, Xiaocong

AU - Nagy, Laura E.

PY - 2008

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N2 - Chronic ethanol consumption dysregulates glucose and lipid homeostasis, is associated with insulin resistance, and alters serum levels of adipokines including adiponectin and tumor necrosis factor-alpha. However, the mechanisms involved in these chronic ethanol-induced pathologies are not fully understood. Adipose tissue has been implicated as an important contributor to chronic ethanol-induced disease states and, therefore, the effects of chronic ethanol feeding in rats on adipocytes has been investigated. Three major functions of the adipocyte include glucose transport, adipokine secretion, and triglyceride breakdown via lipolysis. Included in this chapter are protocols for studying the effect of chronic ethanol feeding on these adipocyte functions.

AB - Chronic ethanol consumption dysregulates glucose and lipid homeostasis, is associated with insulin resistance, and alters serum levels of adipokines including adiponectin and tumor necrosis factor-alpha. However, the mechanisms involved in these chronic ethanol-induced pathologies are not fully understood. Adipose tissue has been implicated as an important contributor to chronic ethanol-induced disease states and, therefore, the effects of chronic ethanol feeding in rats on adipocytes has been investigated. Three major functions of the adipocyte include glucose transport, adipokine secretion, and triglyceride breakdown via lipolysis. Included in this chapter are protocols for studying the effect of chronic ethanol feeding on these adipocyte functions.

KW - Adipocytes/drug effects

KW - Adiponectin/metabolism

KW - Adrenergic beta-Agonists/pharmacology

KW - Animals

KW - Biological Assay/methods

KW - Cell Culture Techniques

KW - Cell Separation

KW - Cells, Cultured

KW - Ethanol/toxicity

KW - Glucose/metabolism

KW - Insulin/metabolism

KW - Isoproterenol/pharmacology

KW - Lipolysis/drug effects

KW - Rats

U2 - 10.1007/978-1-59745-242-7_23

DO - 10.1007/978-1-59745-242-7_23

M3 - Article

C2 - 18369929

SN - 1064-3745

VL - 447

SP - 357

EP - 366

JO - Methods in Molecular Biology (Clifton, N.J.)

JF - Methods in Molecular Biology (Clifton, N.J.)

ER -

Methods to investigate the effects of chronic ethanol on adipocytes

Abstract

Keywords

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