TY - JOUR
T1 - Methylated tissue factor pathway inhibitor 2 (TFPI2) DNA in serum is a biomarker of metastatic melanoma
AU - Nigro, Cristiana Lo
AU - Wang, Hexiao
AU - McHugh, Angela
AU - Lattanzio, Laura
AU - Matin, Rubeta
AU - Harwood, Catherine
AU - Syed, Nelofer
AU - Hatzimichael, Eleftheria
AU - Briasoulis, Evangelos
AU - Merlano, Marco
AU - Evans, Alan
AU - Thompson, Alastair
AU - Leigh, Irene
AU - Fleming, Colin
AU - Inman, Gareth J.
AU - Proby, Charlotte
AU - Crook, Tim
N1 - Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/5
Y1 - 2013/5
N2 - Transcriptional silencing of tissue factor pathway inhibitor 2 (TFPI2) occurs in several human tumors including melanoma. We investigated methylated TFPI2 as a biomarker of metastatic melanoma using qRT-PCR to assess TFPI2 expression and pyrosequencing to analyze CpG island methylation in malignant melanoma cell lines, in benign nevi, in 112 primary and metastatic melanomas, and in serum from 6 healthy individuals and 35 patients: 20 patients with primary and 15 patients with metastatic melanoma. The TFPI2 CpG island is unmethylated in nevi but methylation is associated with metastatic melanoma. Circulating methylated TFPI2 DNA is undetectable in sera from healthy individuals and detectable in sera from patients with primary and metastatic melanomas, but the presence of methylated TFPI2 DNA in serum is strongly associated with metastatic disease (P
AB - Transcriptional silencing of tissue factor pathway inhibitor 2 (TFPI2) occurs in several human tumors including melanoma. We investigated methylated TFPI2 as a biomarker of metastatic melanoma using qRT-PCR to assess TFPI2 expression and pyrosequencing to analyze CpG island methylation in malignant melanoma cell lines, in benign nevi, in 112 primary and metastatic melanomas, and in serum from 6 healthy individuals and 35 patients: 20 patients with primary and 15 patients with metastatic melanoma. The TFPI2 CpG island is unmethylated in nevi but methylation is associated with metastatic melanoma. Circulating methylated TFPI2 DNA is undetectable in sera from healthy individuals and detectable in sera from patients with primary and metastatic melanomas, but the presence of methylated TFPI2 DNA in serum is strongly associated with metastatic disease (P
UR - http://www.scopus.com/inward/record.url?scp=84876670279&partnerID=8YFLogxK
U2 - 10.1038/jid.2012.493
DO - 10.1038/jid.2012.493
M3 - Article
AN - SCOPUS:84876670279
SN - 0022-202X
VL - 133
SP - 1278
EP - 1285
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 5
ER -