Abstract
Radioligand binding studies were performed to determine the effect of various cations on the characteristics of [3H]glibenclamide binding to its high-affinity receptor in rat cerebral cortex membranes. Mg2+ was shown specifically to reduce radioligand binding to membranes pretreated with 5 mM EDTA. CaCl2 enhanced [3H]glibenclamide binding whilst MnCl2, KCl and NaCl were without significant effect. 2 mM MgCl2 induced a statistically significant rightward shift in the dissociation constant for glibenclamide obtained from both saturation and competition studies. These results suggest that Mg2+ may participate in the regulation of the sulphonylurea receptor in the rat cerebral cortex.
| Original language | English |
|---|---|
| Pages (from-to) | 347-351 |
| Number of pages | 5 |
| Journal | European Journal of Pharmacology |
| Volume | 247 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 15 Nov 1993 |
Keywords
- Rats
- Glyburide
- Cerebral Cortex
- Animals
- Receptors, Drug
- Cell Membrane
- Cations, Divalent
- Radioligand Assay
- Magnesium
- Potassium Channels