Microassay for measurement of binding of radiolabelled ligands to cell surface molecules

Jenny M. Woof, Dennis R. Burton (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

An improved technique for measuring the binding of radiolabelled ligands to cell surface molecules has been developed by modification of a procedure using centrifugation through a water-immiscible oil to separate free and cell-bound ligand. It maximises the percentage of ligand bound since cell-bound and free ligand can be separated easily and reproducibly even when very small reaction volumes are used. This permits low levels of ligand radiolabelling and relatively low numbers of cells to be used.

Original languageEnglish
Pages (from-to)205-207
Number of pages3
JournalJournal of immunological methods
Volume111
Issue number2
DOIs
Publication statusPublished - 22 Jul 1988

Keywords

  • Cell Line
  • Centrifugation
  • Humans
  • Immunoglobulin M/metabolism
  • In Vitro Techniques
  • Microchemistry
  • Oils
  • Radioligand Assay/methods
  • Receptors, Cell Surface/metabolism
  • Receptors, Fc/metabolism
  • Receptors, IgG

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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