Abstract
The path that a mesoscopic polarisable particle takes as it flows through a lattice of intensity maxima and minima (optical lattice) depends crucially upon the degree to which it interacts with the lattice. Two particles of dissimilar size, refractive index or even shape will interact in a different manner with such a lattice. Combining this selective interaction with a guiding mechanism has allowed us to achieve lateral separation of particles by all these properties simply by flowing them through an angled optical lattice. We present such particle separation in a variety of three-dimensional optical lattices discussing the importance of parameters such as flow speed, lattice intensity, lattice constant, lattice angle, maxima interconnectivity and flow chamber design. We also present cell sorting with the separation of erythrocytes from lymphocytes and present our flow chamber fabrication methods.
| Original language | English |
|---|---|
| Article number | 01 |
| Pages (from-to) | 1-14 |
| Number of pages | 14 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 5514 |
| DOIs | |
| Publication status | Published - 18 Oct 2004 |
| Event | Optical Trapping and Optical Micromanipulation - Denver, CO, United States Duration: 2 Aug 2004 → 6 Aug 2004 |
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Keywords
- Cell sorting
- Microfluidics
- Optical Fractionation
- Optical Lattice
- Optical Manipulation
- Optical Sorting
Cite this
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Microfluidic optical sorting : Particle selection in an optical lattice. / MacDonald, Michael P.; Neale, Steven; Paterson, Lynn; Riches, Andrew; Spalding, Gabriel C.; Dholakia, Kishan.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 5514, 01, 18.10.2004, p. 1-14.Research output: Contribution to journal › Conference article
TY - JOUR
T1 - Microfluidic optical sorting
T2 - Particle selection in an optical lattice
AU - MacDonald, Michael P.
AU - Neale, Steven
AU - Paterson, Lynn
AU - Riches, Andrew
AU - Spalding, Gabriel C.
AU - Dholakia, Kishan
PY - 2004/10/18
Y1 - 2004/10/18
N2 - The path that a mesoscopic polarisable particle takes as it flows through a lattice of intensity maxima and minima (optical lattice) depends crucially upon the degree to which it interacts with the lattice. Two particles of dissimilar size, refractive index or even shape will interact in a different manner with such a lattice. Combining this selective interaction with a guiding mechanism has allowed us to achieve lateral separation of particles by all these properties simply by flowing them through an angled optical lattice. We present such particle separation in a variety of three-dimensional optical lattices discussing the importance of parameters such as flow speed, lattice intensity, lattice constant, lattice angle, maxima interconnectivity and flow chamber design. We also present cell sorting with the separation of erythrocytes from lymphocytes and present our flow chamber fabrication methods.
AB - The path that a mesoscopic polarisable particle takes as it flows through a lattice of intensity maxima and minima (optical lattice) depends crucially upon the degree to which it interacts with the lattice. Two particles of dissimilar size, refractive index or even shape will interact in a different manner with such a lattice. Combining this selective interaction with a guiding mechanism has allowed us to achieve lateral separation of particles by all these properties simply by flowing them through an angled optical lattice. We present such particle separation in a variety of three-dimensional optical lattices discussing the importance of parameters such as flow speed, lattice intensity, lattice constant, lattice angle, maxima interconnectivity and flow chamber design. We also present cell sorting with the separation of erythrocytes from lymphocytes and present our flow chamber fabrication methods.
KW - Cell sorting
KW - Microfluidics
KW - Optical Fractionation
KW - Optical Lattice
KW - Optical Manipulation
KW - Optical Sorting
UR - http://www.scopus.com/inward/record.url?scp=13244284656&partnerID=8YFLogxK
U2 - 10.1117/12.559522
DO - 10.1117/12.559522
M3 - Conference article
AN - SCOPUS:13244284656
VL - 5514
SP - 1
EP - 14
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
SN - 0277-786X
M1 - 01
ER -