Microultrasound characterisation of ex vivo porcine tissue for ultrasound capsule endoscopy

H. S. Lay (Lead / Corresponding author), B. F. Cox, M. Sunoqrot, C. E. M. Démoré, I. Näthke, T. Gomez, S. Cochran

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    10 Citations (Scopus)
    157 Downloads (Pure)

    Abstract

    Gastrointestinal (GI) disease development and progression is often characterised by cellular and tissue architectural changes within the mucosa and sub-mucosa layers. Current clinical capsule endoscopy and other approaches are heavily reliant on optical techniques which cannot detect disease progression below the surface layer of the tissue. To enhance the ability of clinicians to detect cellular changes earlier and more confidently, both quantitative and qualitative microultrasound (μUS) techniques are investigated in healthy ex vivo porcine GI tissue. This work is based on the use of single-element, focussed μUS transducers made with micromoulded piezocomposite operating at around 48 MHz. To explore the possibility that μUS can detect Crohn's disease and other inflammatory bowel diseases, ex vivo porcine small bowel tissue samples were cannulised and perfused with phosphate-buffered saline followed by various dilutions of polystyrene microspheres. Comparison with fluorescent imaging showed that the microspheres had infiltrated the microvasculature of the samples and that μUS was able to successfully detect this as a mimic of inflammation. Samples without microspheres were analysed using quantitative ultrasound to assess mechanical properties. Attenuation coefficients of 1.78 ± 0.66 dB/mm and 1.92 ± 0.77 dB/mm were obtained from reference samples which were surgically separated from the muscle layer. Six intact samples were segmented using a software algorithm and the acoustic impedance, Z, for varying tissue thicknesses, and backscattering coefficient, BSC, were calculated using the reference attenuation values and tabulated.

    Original languageEnglish
    Article number012003
    Pages (from-to)1-12
    Number of pages12
    JournalJournal of Physics: Conference Series
    Volume797
    Issue number1
    DOIs
    Publication statusPublished - 18 Jan 2017

    ASJC Scopus subject areas

    • General Physics and Astronomy

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