Migration-stimulating factor as a novel biomarker in salivary gland tumours

Lateef E. Aljorani, Agnes Bankfalvi, Frank A. Carey, Koji Harada, Go Ohe, Sarah J. Jones, Ian R. Ellis, Seth L. Schor, Ana M. Schor

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

BACKGROUND: The identification of novel stratification biomarkers would benefit the clinical management of patients with salivary gland tumours. Migration-stimulating factor (MSF) is a potent stimulator of cell invasion, matrix remodelling and angiogenesis. The aim of this study was to determine whether MSF was expressed in salivary gland tumours and its potential value as a diagnostic biomarker.

METHODS: Paraffin-embedded archival specimens of small salivary gland tumours were stained with an MSF-specific antibody. The specimens included 27 malignant and seven benign tumours; histologically normal salivary gland adjacent to the tumour was present in 16 specimens. MSF expression was assessed by consensus of 2-4 independent observers according to various indices, including 'overall MSF grade', 'percentage of area stained' and 'intensity of the staining'. The motogenic effect of MSF on a salivary gland tumour cell line, HSG, was examined in the transmembrane assay.

RESULTS: Overall MSF expression increased significantly in a step-wise fashion from normal salivary gland to benign and malignant tumours (P = 0.04-0.0001); with moderate/strong positive specimens representing 6%, 33% and 74% of the normal, benign and malignant specimens, respectively. MSF was heterogeneously expressed in both carcinoma and stromal cell compartments, its expression being higher in malignant than benign tumours regarding various MSF indices. In tissue culture studies, exogenous MSF stimulated the migration of HSG cells.

CONCLUSIONS: These immunohistochemical and functional studies suggest that MSF expression is a potentially useful biomarker of salivary gland tumour progression.

Original languageEnglish
Pages (from-to)747-754
Number of pages8
JournalJournal of Oral Pathology and Medicine
Volume40
Issue number10
DOIs
Publication statusPublished - Nov 2011

Keywords

  • benign tumours
  • cancer
  • disease progression
  • MSF
  • salivary gland tumours
  • ENDOTHELIAL-CELLS
  • ANGIOGENESIS
  • FIBRONECTIN
  • FIBROBLASTS
  • EXPRESSION
  • CARCINOMA

Cite this

Aljorani, Lateef E. ; Bankfalvi, Agnes ; Carey, Frank A. ; Harada, Koji ; Ohe, Go ; Jones, Sarah J. ; Ellis, Ian R. ; Schor, Seth L. ; Schor, Ana M. / Migration-stimulating factor as a novel biomarker in salivary gland tumours. In: Journal of Oral Pathology and Medicine. 2011 ; Vol. 40, No. 10. pp. 747-754.
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abstract = "BACKGROUND: The identification of novel stratification biomarkers would benefit the clinical management of patients with salivary gland tumours. Migration-stimulating factor (MSF) is a potent stimulator of cell invasion, matrix remodelling and angiogenesis. The aim of this study was to determine whether MSF was expressed in salivary gland tumours and its potential value as a diagnostic biomarker.METHODS: Paraffin-embedded archival specimens of small salivary gland tumours were stained with an MSF-specific antibody. The specimens included 27 malignant and seven benign tumours; histologically normal salivary gland adjacent to the tumour was present in 16 specimens. MSF expression was assessed by consensus of 2-4 independent observers according to various indices, including 'overall MSF grade', 'percentage of area stained' and 'intensity of the staining'. The motogenic effect of MSF on a salivary gland tumour cell line, HSG, was examined in the transmembrane assay.RESULTS: Overall MSF expression increased significantly in a step-wise fashion from normal salivary gland to benign and malignant tumours (P = 0.04-0.0001); with moderate/strong positive specimens representing 6{\%}, 33{\%} and 74{\%} of the normal, benign and malignant specimens, respectively. MSF was heterogeneously expressed in both carcinoma and stromal cell compartments, its expression being higher in malignant than benign tumours regarding various MSF indices. In tissue culture studies, exogenous MSF stimulated the migration of HSG cells.CONCLUSIONS: These immunohistochemical and functional studies suggest that MSF expression is a potentially useful biomarker of salivary gland tumour progression.",
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Aljorani, LE, Bankfalvi, A, Carey, FA, Harada, K, Ohe, G, Jones, SJ, Ellis, IR, Schor, SL & Schor, AM 2011, 'Migration-stimulating factor as a novel biomarker in salivary gland tumours', Journal of Oral Pathology and Medicine, vol. 40, no. 10, pp. 747-754. https://doi.org/10.1111/j.1600-0714.2011.01044.x

Migration-stimulating factor as a novel biomarker in salivary gland tumours. / Aljorani, Lateef E.; Bankfalvi, Agnes; Carey, Frank A.; Harada, Koji; Ohe, Go; Jones, Sarah J.; Ellis, Ian R.; Schor, Seth L.; Schor, Ana M.

In: Journal of Oral Pathology and Medicine, Vol. 40, No. 10, 11.2011, p. 747-754.

Research output: Contribution to journalArticle

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T1 - Migration-stimulating factor as a novel biomarker in salivary gland tumours

AU - Aljorani, Lateef E.

AU - Bankfalvi, Agnes

AU - Carey, Frank A.

AU - Harada, Koji

AU - Ohe, Go

AU - Jones, Sarah J.

AU - Ellis, Ian R.

AU - Schor, Seth L.

AU - Schor, Ana M.

N1 - © 2011 John Wiley & Sons A/S.

PY - 2011/11

Y1 - 2011/11

N2 - BACKGROUND: The identification of novel stratification biomarkers would benefit the clinical management of patients with salivary gland tumours. Migration-stimulating factor (MSF) is a potent stimulator of cell invasion, matrix remodelling and angiogenesis. The aim of this study was to determine whether MSF was expressed in salivary gland tumours and its potential value as a diagnostic biomarker.METHODS: Paraffin-embedded archival specimens of small salivary gland tumours were stained with an MSF-specific antibody. The specimens included 27 malignant and seven benign tumours; histologically normal salivary gland adjacent to the tumour was present in 16 specimens. MSF expression was assessed by consensus of 2-4 independent observers according to various indices, including 'overall MSF grade', 'percentage of area stained' and 'intensity of the staining'. The motogenic effect of MSF on a salivary gland tumour cell line, HSG, was examined in the transmembrane assay.RESULTS: Overall MSF expression increased significantly in a step-wise fashion from normal salivary gland to benign and malignant tumours (P = 0.04-0.0001); with moderate/strong positive specimens representing 6%, 33% and 74% of the normal, benign and malignant specimens, respectively. MSF was heterogeneously expressed in both carcinoma and stromal cell compartments, its expression being higher in malignant than benign tumours regarding various MSF indices. In tissue culture studies, exogenous MSF stimulated the migration of HSG cells.CONCLUSIONS: These immunohistochemical and functional studies suggest that MSF expression is a potentially useful biomarker of salivary gland tumour progression.

AB - BACKGROUND: The identification of novel stratification biomarkers would benefit the clinical management of patients with salivary gland tumours. Migration-stimulating factor (MSF) is a potent stimulator of cell invasion, matrix remodelling and angiogenesis. The aim of this study was to determine whether MSF was expressed in salivary gland tumours and its potential value as a diagnostic biomarker.METHODS: Paraffin-embedded archival specimens of small salivary gland tumours were stained with an MSF-specific antibody. The specimens included 27 malignant and seven benign tumours; histologically normal salivary gland adjacent to the tumour was present in 16 specimens. MSF expression was assessed by consensus of 2-4 independent observers according to various indices, including 'overall MSF grade', 'percentage of area stained' and 'intensity of the staining'. The motogenic effect of MSF on a salivary gland tumour cell line, HSG, was examined in the transmembrane assay.RESULTS: Overall MSF expression increased significantly in a step-wise fashion from normal salivary gland to benign and malignant tumours (P = 0.04-0.0001); with moderate/strong positive specimens representing 6%, 33% and 74% of the normal, benign and malignant specimens, respectively. MSF was heterogeneously expressed in both carcinoma and stromal cell compartments, its expression being higher in malignant than benign tumours regarding various MSF indices. In tissue culture studies, exogenous MSF stimulated the migration of HSG cells.CONCLUSIONS: These immunohistochemical and functional studies suggest that MSF expression is a potentially useful biomarker of salivary gland tumour progression.

KW - benign tumours

KW - cancer

KW - disease progression

KW - MSF

KW - salivary gland tumours

KW - ENDOTHELIAL-CELLS

KW - ANGIOGENESIS

KW - FIBRONECTIN

KW - FIBROBLASTS

KW - EXPRESSION

KW - CARCINOMA

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DO - 10.1111/j.1600-0714.2011.01044.x

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JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

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