Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT

Ian R. Ellis, Sarah J. Jones, Yvonne Lindsay, Go Ohe, Ana M. Schor, Seth L. Schor, Nick R. Leslie

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    Abstract

    The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits Ala activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT. (C) 2010 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)1655-1659
    Number of pages5
    JournalCellular Signalling
    Volume22
    Issue number11
    DOIs
    Publication statusPublished - Nov 2010

    Keywords

    • AKT
    • MSF
    • Kinase
    • Cell migration
    • Phosphoinositide 3-kinase
    • PROTEIN-KINASE B/AKT
    • CELL-MIGRATION
    • ENDOTHELIAL-CELLS
    • EGF RECEPTOR
    • IN-VITRO
    • CHEMOTAXIS
    • INSULIN
    • CANCER
    • FIBRONECTIN
    • INVASION

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