Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT

Ian R. Ellis; Sarah J. Jones; Yvonne Lindsay; Go Ohe; Ana M. Schor; Seth L. Schor; Nick R. Leslie

doi:10.1016/j.cellsig.2010.06.005

Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT

Ian R. Ellis, Sarah J. Jones, Yvonne Lindsay, Go Ohe, Ana M. Schor, Seth L. Schor, Nick R. Leslie

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits Ala activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT. (C) 2010 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	1655-1659
Number of pages	5
Journal	Cellular Signalling
Volume	22
Issue number	11
DOIs	https://doi.org/10.1016/j.cellsig.2010.06.005
Publication status	Published - Nov 2010

Keywords

AKT
MSF
Kinase
Cell migration
Phosphoinositide 3-kinase
PROTEIN-KINASE B/AKT
CELL-MIGRATION
ENDOTHELIAL-CELLS
EGF RECEPTOR
IN-VITRO
CHEMOTAXIS
INSULIN
CANCER
FIBRONECTIN
INVASION

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cellsig.2010.06.005

Aref#d: 21320. Antagonism of PI 3-Kinase Signalling by PTEN and SHIP2 (Programme Grant)
Downes, P. (Investigator) & Leslie, N. (Investigator)
Medical Research Council
1/10/09 → 30/09/14
Project: Research

Cite this

@article{a92815aa53e64572a62b85a9de8a8889,

title = "Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT",

abstract = "The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits Ala activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT. (C) 2010 Elsevier Inc. All rights reserved.",

keywords = "AKT, MSF, Kinase, Cell migration, Phosphoinositide 3-kinase, PROTEIN-KINASE B/AKT, CELL-MIGRATION, ENDOTHELIAL-CELLS, EGF RECEPTOR, IN-VITRO, CHEMOTAXIS, INSULIN, CANCER, FIBRONECTIN, INVASION",

author = "Ellis, {Ian R.} and Jones, {Sarah J.} and Yvonne Lindsay and Go Ohe and Schor, {Ana M.} and Schor, {Seth L.} and Leslie, {Nick R.}",

year = "2010",

month = nov,

doi = "10.1016/j.cellsig.2010.06.005",

language = "English",

volume = "22",

pages = "1655--1659",

journal = "Cellular Signalling",

issn = "0898-6568",

publisher = "Elsevier",

number = "11",

}

TY - JOUR

T1 - Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT

AU - Ellis, Ian R.

AU - Jones, Sarah J.

AU - Lindsay, Yvonne

AU - Ohe, Go

AU - Schor, Ana M.

AU - Schor, Seth L.

AU - Leslie, Nick R.

PY - 2010/11

Y1 - 2010/11

N2 - The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits Ala activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT. (C) 2010 Elsevier Inc. All rights reserved.

AB - The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits Ala activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT. (C) 2010 Elsevier Inc. All rights reserved.

KW - AKT

KW - MSF

KW - Kinase

KW - Cell migration

KW - Phosphoinositide 3-kinase

KW - PROTEIN-KINASE B/AKT

KW - CELL-MIGRATION

KW - ENDOTHELIAL-CELLS

KW - EGF RECEPTOR

KW - IN-VITRO

KW - CHEMOTAXIS

KW - INSULIN

KW - CANCER

KW - FIBRONECTIN

KW - INVASION

U2 - 10.1016/j.cellsig.2010.06.005

DO - 10.1016/j.cellsig.2010.06.005

M3 - Article

C2 - 20600851

SN - 0898-6568

VL - 22

SP - 1655

EP - 1659

JO - Cellular Signalling

JF - Cellular Signalling

IS - 11

ER -

Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT

Abstract

Keywords

UN SDGs

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Projects

Aref#d: 21320. Antagonism of PI 3-Kinase Signalling by PTEN and SHIP2 (Programme Grant)

Cite this