Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node

Yang Zhang, Theodore L. Roth, Elizabeth E. Gray, Hsin Chen, Lauren B. Rodda, Yin Liang, Patrick Ventura, Saul Villeda, Paul R. Crocker, Jason G. Cyster (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Lymph nodes (LNs) contain innate-like lymphocytes that survey the subcapsular sinus (SCS) and associated macrophages for pathogen entry. The factors promoting this surveillance behavior have not been defined. Here we report that IL7(Rhi)Ccr6(+) lymphocytes in mouse LNs rapidly produce IL17 upon bacterial and fungal challenge. We show that these innate-like lymphocytes are mostly LN resident. Ccr6 is required for their accumulation near the SCS and for efficient IL17 induction. Migration into the SCS intrinsically requires S1pr1 whereas movement from the sinus into the parenchyma involves the integrin LFA1 and its ligand ICAM1. CD169, a sialic acid-binding lectin, helps retain the cells within the sinus, preventing their loss in lymph flow. These findings establish a role for Ccr6 in augmenting innate-like lymphocyte responses to lymph-borne pathogens, and they define requirements for cell movement between parenchyma and SCS in what we speculate is a program of immune surveillance that helps achieve LN barrier immunity.

Original languageEnglish
Article numbere18156
Pages (from-to)1-29
Number of pages29
JournaleLife
Volume5
DOIs
Publication statusPublished - 3 Aug 2016

Fingerprint

Lymphocytes
Pathogens
Adhesives
Cues
Lymph Nodes
Lymph
Interleukin-7
Macrophages
N-Acetylneuraminic Acid
Lectins
Integrins
Cell Movement
Immunity
Cells
Ligands

Keywords

  • Immunology
  • CD169
  • IL17
  • Innate like lymphocytes
  • Lymph node
  • Migration
  • Mouse
  • SCS Macrophage

Cite this

Zhang, Y., Roth, T. L., Gray, E. E., Chen, H., Rodda, L. B., Liang, Y., ... Cyster, J. G. (2016). Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node. eLife, 5, 1-29. [e18156]. https://doi.org/10.7554/eLife.18156
Zhang, Yang ; Roth, Theodore L. ; Gray, Elizabeth E. ; Chen, Hsin ; Rodda, Lauren B. ; Liang, Yin ; Ventura, Patrick ; Villeda, Saul ; Crocker, Paul R. ; Cyster, Jason G. / Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node. In: eLife. 2016 ; Vol. 5. pp. 1-29.
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abstract = "Lymph nodes (LNs) contain innate-like lymphocytes that survey the subcapsular sinus (SCS) and associated macrophages for pathogen entry. The factors promoting this surveillance behavior have not been defined. Here we report that IL7(Rhi)Ccr6(+) lymphocytes in mouse LNs rapidly produce IL17 upon bacterial and fungal challenge. We show that these innate-like lymphocytes are mostly LN resident. Ccr6 is required for their accumulation near the SCS and for efficient IL17 induction. Migration into the SCS intrinsically requires S1pr1 whereas movement from the sinus into the parenchyma involves the integrin LFA1 and its ligand ICAM1. CD169, a sialic acid-binding lectin, helps retain the cells within the sinus, preventing their loss in lymph flow. These findings establish a role for Ccr6 in augmenting innate-like lymphocyte responses to lymph-borne pathogens, and they define requirements for cell movement between parenchyma and SCS in what we speculate is a program of immune surveillance that helps achieve LN barrier immunity.",
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Zhang, Y, Roth, TL, Gray, EE, Chen, H, Rodda, LB, Liang, Y, Ventura, P, Villeda, S, Crocker, PR & Cyster, JG 2016, 'Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node', eLife, vol. 5, e18156, pp. 1-29. https://doi.org/10.7554/eLife.18156

Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node. / Zhang, Yang; Roth, Theodore L.; Gray, Elizabeth E.; Chen, Hsin; Rodda, Lauren B.; Liang, Yin; Ventura, Patrick; Villeda, Saul; Crocker, Paul R.; Cyster, Jason G. (Lead / Corresponding author).

In: eLife, Vol. 5, e18156, 03.08.2016, p. 1-29.

Research output: Contribution to journalArticle

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T1 - Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node

AU - Zhang, Yang

AU - Roth, Theodore L.

AU - Gray, Elizabeth E.

AU - Chen, Hsin

AU - Rodda, Lauren B.

AU - Liang, Yin

AU - Ventura, Patrick

AU - Villeda, Saul

AU - Crocker, Paul R.

AU - Cyster, Jason G.

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AB - Lymph nodes (LNs) contain innate-like lymphocytes that survey the subcapsular sinus (SCS) and associated macrophages for pathogen entry. The factors promoting this surveillance behavior have not been defined. Here we report that IL7(Rhi)Ccr6(+) lymphocytes in mouse LNs rapidly produce IL17 upon bacterial and fungal challenge. We show that these innate-like lymphocytes are mostly LN resident. Ccr6 is required for their accumulation near the SCS and for efficient IL17 induction. Migration into the SCS intrinsically requires S1pr1 whereas movement from the sinus into the parenchyma involves the integrin LFA1 and its ligand ICAM1. CD169, a sialic acid-binding lectin, helps retain the cells within the sinus, preventing their loss in lymph flow. These findings establish a role for Ccr6 in augmenting innate-like lymphocyte responses to lymph-borne pathogens, and they define requirements for cell movement between parenchyma and SCS in what we speculate is a program of immune surveillance that helps achieve LN barrier immunity.

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