TY - JOUR
T1 - miRNA analysis of formalin-fixed squamous cell carcinomas of the tongue is affected by age of the samples
AU - Rentoft, M
AU - Fahlén, J
AU - Coates, P J
AU - Laurell, G
AU - Sjöström, B
AU - Rydén, P
AU - Nylander, K
PY - 2011
Y1 - 2011
N2 - Global miRNA expression arrays were used for analysis of 836 miRNAs in formalin-fixed paraffin-embedded samples from 21 tongue cancer patients and 8 controls. Samples had been stored for one to eleven years. Results separated tumour samples from controls, however, the largest variation was correlated to sample storage time, detectable already after one year. With the use of a linear regression model we could adjust for the storage-dependent effect, leading to the identification of 54 differentially expressed miRNAs in tongue cancer, compared to 16 when using standard normalization, including up-regulation of a novel miRNA, miR-424.
AB - Global miRNA expression arrays were used for analysis of 836 miRNAs in formalin-fixed paraffin-embedded samples from 21 tongue cancer patients and 8 controls. Samples had been stored for one to eleven years. Results separated tumour samples from controls, however, the largest variation was correlated to sample storage time, detectable already after one year. With the use of a linear regression model we could adjust for the storage-dependent effect, leading to the identification of 54 differentially expressed miRNAs in tongue cancer, compared to 16 when using standard normalization, including up-regulation of a novel miRNA, miR-424.
U2 - 10.3892/ijo_00000824
DO - 10.3892/ijo_00000824
M3 - Article
C2 - 21109926
SN - 1791-2423
VL - 38
SP - 61
EP - 69
JO - International Journal of Oncology
JF - International Journal of Oncology
IS - 1
ER -