miRNA analysis of formalin-fixed squamous cell carcinomas of the tongue is affected by age of the samples

M Rentoft, J Fahlén, P J Coates, G Laurell, B Sjöström, P Rydén, K Nylander

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)

    Abstract

    Global miRNA expression arrays were used for analysis of 836 miRNAs in formalin-fixed paraffin-embedded samples from 21 tongue cancer patients and 8 controls. Samples had been stored for one to eleven years. Results separated tumour samples from controls, however, the largest variation was correlated to sample storage time, detectable already after one year. With the use of a linear regression model we could adjust for the storage-dependent effect, leading to the identification of 54 differentially expressed miRNAs in tongue cancer, compared to 16 when using standard normalization, including up-regulation of a novel miRNA, miR-424.
    Original languageEnglish
    Pages (from-to)61-69
    Number of pages9
    JournalInternational Journal of Oncology
    Volume38
    Issue number1
    DOIs
    Publication statusPublished - 2011

    Fingerprint Dive into the research topics of 'miRNA analysis of formalin-fixed squamous cell carcinomas of the tongue is affected by age of the samples'. Together they form a unique fingerprint.

    Cite this