Mobilisation of stored calcium in the neck region of human sperm: a mechanism for regulation of flagellar activity

Kweku Bedu-Addo, Sarah Costello, Claire Harper, Gisela Machado-Oliveira, Linda Lefievre, Christopher Ford, Christopher Barratt, Stephen Publicover

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    52 Citations (Scopus)

    Abstract

    Calcium signalling plays a pivotal role in sperm physiology, being intimately involved in the regulation of acrosome reaction, chemotaxis and hyperactivation. Here we describe briefly the mechanisms of calcium regulation in somatic cells and the ways in which these mechanisms have been adapted to function in mature spermatozoa. We then consider recent data from this and other laboratories on the responses of sperm to three compounds: progesterone and nitric oxide (both products of the cumulus oophorus) and 4-aminopyridine. All of these compounds induce calcium signals in the posterior sperm head and neck region and, when applied at appropriate concentrations, modify flagellar activity, causing asymmetric bending of the proximal flagellum. We argue that these effects reflect a common mode of action, mobilisation of calcium stored in the sperm neck region. Finally we consider the nature of calcium signalling pathways in sperm. We suggest that this highly specialised and extremely polarised cell, though working with the same calcium signalling 'tools' as those of somatic cells, employs them to generate unusually 'hard-wired' calcium signals that do not act to integrate stimuli. 'Leakage' between these calcium signalling pathways will generate inappropriate responses, compromising functioning of the cell.

    Original languageEnglish
    Pages (from-to)615-626
    Number of pages12
    JournalInternational Journal of Developmental Biology
    Volume52
    Issue number5-6
    DOIs
    Publication statusPublished - 2008

    Keywords

    • Sperm
    • Calcium
    • Nitric oxide
    • Calcium store
    • Hyperactivation
    • Inositol 1,4,5-Trisphosphate receptor
    • Secretory pathway CA2+ atpase
    • Induced acrosome reaction
    • Sea urchin spermatozoa
    • Bovine serum albumin
    • Plasma membrane
    • Mammalian sperm
    • Hyperactivated motility

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