Molecular analysis of the microbiome in colorectal cancer

Fiona Clegg, Susan H. Berry, Richard Hansen, Georgina L. Hold (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The human gut microbiota plays a major role in the development of colorectal cancer (CRC). Many studies have attempted to define links between microbiota residents, their function and disease development. We now have incredible molecular tools to allow us to study the gut microbiome however in order to make best use of these sophisticated approaches we need to ensure that samples are collected and processed using standardized and reproducible protocols. Here we provide an overview of molecular analysis methods and describe protocols for collecting and processing clinical samples for subsequent microbiome analysis.

Original languageEnglish
Title of host publicationColorectal Cancer
Subtitle of host publicationMethods and Protocols
EditorsJean-François Beaulieu
PublisherHumana Press Inc.
Chapter8
Pages139-153
Number of pages15
ISBN (Electronic)978-1-4939-7765-9
ISBN (Print)978-1-4939-9278-2, 978-1-4939-7764-2
DOIs
Publication statusPublished - 27 Mar 2018

Publication series

NameMethods in Molecular Biology
Volume1765
ISSN (Print)1064-3745

Keywords

  • Bacteria
  • Biopsy
  • Clinical samples
  • Colorectal cancer
  • DNA
  • Fungi
  • Microbial diversity
  • Microbiome
  • Molecular microbiology
  • RNA
  • Stool

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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