Molecular analysis of the microbiome in colorectal cancer

Fiona Clegg, Susan H. Berry, Richard Hansen, Georgina L. Hold (Lead / Corresponding author)

    Research output: Chapter in Book/Report/Conference proceedingChapter

    2 Citations (Scopus)

    Abstract

    The human gut microbiota plays a major role in the development of colorectal cancer (CRC). Many studies have attempted to define links between microbiota residents, their function and disease development. We now have incredible molecular tools to allow us to study the gut microbiome however in order to make best use of these sophisticated approaches we need to ensure that samples are collected and processed using standardized and reproducible protocols. Here we provide an overview of molecular analysis methods and describe protocols for collecting and processing clinical samples for subsequent microbiome analysis.

    Original languageEnglish
    Title of host publicationColorectal Cancer
    Subtitle of host publicationMethods and Protocols
    EditorsJean-François Beaulieu
    PublisherHumana Press
    Chapter8
    Pages139-153
    Number of pages15
    ISBN (Electronic)978-1-4939-7765-9
    ISBN (Print)978-1-4939-9278-2, 978-1-4939-7764-2
    DOIs
    Publication statusPublished - 27 Mar 2018

    Publication series

    NameMethods in Molecular Biology
    Volume1765
    ISSN (Print)1064-3745

    Keywords

    • Bacteria
    • Biopsy
    • Clinical samples
    • Colorectal cancer
    • DNA
    • Fungi
    • Microbial diversity
    • Microbiome
    • Molecular microbiology
    • RNA
    • Stool

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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