Molecular analysis of the microbiome in colorectal cancer

Fiona Clegg; Susan H. Berry; Richard Hansen; Georgina L. Hold

doi:10.1007/978-1-4939-7765-9_8

Molecular analysis of the microbiome in colorectal cancer

Fiona Clegg
, Susan H. Berry
, Richard Hansen
, Georgina L. Hold (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

The human gut microbiota plays a major role in the development of colorectal cancer (CRC). Many studies have attempted to define links between microbiota residents, their function and disease development. We now have incredible molecular tools to allow us to study the gut microbiome however in order to make best use of these sophisticated approaches we need to ensure that samples are collected and processed using standardized and reproducible protocols. Here we provide an overview of molecular analysis methods and describe protocols for collecting and processing clinical samples for subsequent microbiome analysis.

Original language	English
Title of host publication	Colorectal Cancer
Subtitle of host publication	Methods and Protocols
Editors	Jean-François Beaulieu
Publisher	Humana Press
Chapter	8
Pages	139-153
Number of pages	15
ISBN (Electronic)	978-1-4939-7765-9
ISBN (Print)	978-1-4939-9278-2, 978-1-4939-7764-2
DOIs	https://doi.org/10.1007/978-1-4939-7765-9_8
Publication status	Published - 27 Mar 2018

Publication series

Name	Methods in Molecular Biology
Volume	1765
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bacteria
Biopsy
Clinical samples
Colorectal cancer
DNA
Fungi
Microbial diversity
Microbiome
Molecular microbiology
RNA
Stool

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-7765-9_8

Cite this

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title = "Molecular analysis of the microbiome in colorectal cancer",

abstract = "The human gut microbiota plays a major role in the development of colorectal cancer (CRC). Many studies have attempted to define links between microbiota residents, their function and disease development. We now have incredible molecular tools to allow us to study the gut microbiome however in order to make best use of these sophisticated approaches we need to ensure that samples are collected and processed using standardized and reproducible protocols. Here we provide an overview of molecular analysis methods and describe protocols for collecting and processing clinical samples for subsequent microbiome analysis.",

keywords = "Bacteria, Biopsy, Clinical samples, Colorectal cancer, DNA, Fungi, Microbial diversity, Microbiome, Molecular microbiology, RNA, Stool",

author = "Fiona Clegg and Berry, \{Susan H.\} and Richard Hansen and Hold, \{Georgina L.\}",

note = "Funding Information: This work was supported by grants from The Chief Scientist Office, Friends of Anchor, NHS Grampian Endowments, NHS Grampian Gastroenterology Endowment funds as well as donations from CRC patients. Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",

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AU - Clegg, Fiona

AU - Berry, Susan H.

AU - Hansen, Richard

AU - Hold, Georgina L.

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N2 - The human gut microbiota plays a major role in the development of colorectal cancer (CRC). Many studies have attempted to define links between microbiota residents, their function and disease development. We now have incredible molecular tools to allow us to study the gut microbiome however in order to make best use of these sophisticated approaches we need to ensure that samples are collected and processed using standardized and reproducible protocols. Here we provide an overview of molecular analysis methods and describe protocols for collecting and processing clinical samples for subsequent microbiome analysis.

AB - The human gut microbiota plays a major role in the development of colorectal cancer (CRC). Many studies have attempted to define links between microbiota residents, their function and disease development. We now have incredible molecular tools to allow us to study the gut microbiome however in order to make best use of these sophisticated approaches we need to ensure that samples are collected and processed using standardized and reproducible protocols. Here we provide an overview of molecular analysis methods and describe protocols for collecting and processing clinical samples for subsequent microbiome analysis.

KW - Bacteria

KW - Biopsy

KW - Clinical samples

KW - Colorectal cancer

KW - DNA

KW - Fungi

KW - Microbial diversity

KW - Microbiome

KW - Molecular microbiology

KW - RNA

KW - Stool

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DO - 10.1007/978-1-4939-7765-9_8

M3 - Chapter

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AN - SCOPUS:85044667321

SN - 978-1-4939-9278-2

SN - 978-1-4939-7764-2

T3 - Methods in Molecular Biology

SP - 139

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BT - Colorectal Cancer

A2 - Beaulieu, Jean-François

PB - Humana Press

ER -

Molecular analysis of the microbiome in colorectal cancer

Abstract

Publication series

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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