Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC

Cynthia Tallant, Erica Valentini, Oleg Fedorov, Lois Overvoorde, Fleur M. Ferguson, Panagis Filippakopoulos, Dmitri I. Svergun, Stefan Knapp, Alessio Ciulli (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

Binding of the chromatin remodeling complex NoRC to RNA complementary to the rDNA promoter mediates transcriptional repression. TIP5, the largest subunit of NoRC, is involved in recruitment to rDNA by interactions with promoter-bound TTF-I, pRNA, and acetylation of H4K16. TIP5 domains that recognize posttranslational modifications on histones are essential for recruitment of NoRC to chromatin, but how these reader modules recognize site-specific histone tails has remained elusive. Here, we report crystal structures of PHD zinc finger and bromodomains from human TIP5 and BAZ2B in free form and bound to H3 and/or H4 histones. PHD finger functions as an independent structural module in recognizing unmodified H3 histone tails, and the bromodomain prefers H3 and H4 acetylation marks followed by a key basic residue, KacXXR. Further low-resolution analyses of PHD-bromodomain modules provide molecular insights into their trans histone tail recognition, required for nucleosome recruitment and transcriptional repression of the NoRC complex.

Original languageEnglish
Pages (from-to)80-92
Number of pages13
JournalStructure
Volume23
Issue number1
DOIs
Publication statusPublished - 6 Jan 2015

Keywords

  • Amino acid sequence
  • Chromatin assembly and disassembly
  • Chromosomal proteins, Non-histone
  • Histones
  • Humans
  • Models, Molecular
  • Molecular sequence data
  • Protein binding
  • Protein interaction domains and motifs
  • Protein structure, Quaternary
  • Sequence homology, Amino acid
  • Zinc fingers

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