Molecular characterization of nitrogen fixing microsymbionts from root nodules of Vachellia (Acacia) jacquemontii, a native legume from the Thar Desert of India

Indu Singh Sankhla, Nisha Tak, Raju Ram Meghwal, Sunil Choudhary, Alkesh Tak, Sonam Rathi, Janet I. Sprent, Euan K. James (Lead / Corresponding author), Hukam Singh Gehlot (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Aims: To describe the structure of nodules of Vachellia (Acacia) jacquemontii, and to characterise the rhizobia that occupy them. 

    Methods: Light and electron microscopy were used to analyse nodules. Rhizobia were characterised using their 16S rRNA, housekeeping and symbiosis-related gene sequences. 

    Results: Nodules of V. jacquemontii were typical of all other described mimosoid legumes. All 73 of the isolates were strains of Ensifer, and concatenated phylogenetic analysis of their housekeeping genes (rrs, recA, atpD, glnII and dnaK) suggested that they are novel, forming separate lineages close to E. saheli. The phylogenies of the symbiosis-essential genes nodA and nifH were inconsistent with the housekeeping phylogenies. The nodA sequences of most isolates were close to that of E. arboris HAMBI 1552T, but the nifH gene was found to be related to that of E. kostiensis HAMBI 1489T. All the tested Ensifer strains, except for AJ24, were found to be capable of nodulating other species of Vachellia as well as native Indian Mimosa and Prosopis spp. 

    Conclusions: Stressful conditions caused by the alkaline soil of the Thar Desert have resulted in V. jacquemontii being nodulated by diverse and promiscuous Ensifer species that are capable of nodulating other native members of the tribe Mimoseae.

    Original languageEnglish
    Pages (from-to)21-40
    Number of pages20
    JournalPlant and Soil
    Volume410
    Issue number1
    Early online date29 Feb 2016
    DOIs
    Publication statusPublished - Jan 2017

    Fingerprint

    Vachellia
    Ensifer
    Thar Desert
    microsymbionts
    root nodules
    Acacia
    legumes
    desert
    India
    Rhizobium
    symbiosis
    gene
    nitrogen
    phylogeny
    rhizobacterium
    Prosopis
    Mimosa
    alkaline soils
    light microscopy
    electron microscopy

    Keywords

    • Ensifer
    • Multi Locus Sequence Analysis (MLSA)
    • nifH
    • nodA
    • rhizobia
    • Thar Desert
    • Vachellia (Acacia) jacquemontii

    Cite this

    Sankhla, Indu Singh ; Tak, Nisha ; Meghwal, Raju Ram ; Choudhary, Sunil ; Tak, Alkesh ; Rathi, Sonam ; Sprent, Janet I. ; James, Euan K. ; Gehlot, Hukam Singh. / Molecular characterization of nitrogen fixing microsymbionts from root nodules of Vachellia (Acacia) jacquemontii, a native legume from the Thar Desert of India. In: Plant and Soil. 2017 ; Vol. 410, No. 1. pp. 21-40.
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    title = "Molecular characterization of nitrogen fixing microsymbionts from root nodules of Vachellia (Acacia) jacquemontii, a native legume from the Thar Desert of India",
    abstract = "Aims: To describe the structure of nodules of Vachellia (Acacia) jacquemontii, and to characterise the rhizobia that occupy them. Methods: Light and electron microscopy were used to analyse nodules. Rhizobia were characterised using their 16S rRNA, housekeeping and symbiosis-related gene sequences. Results: Nodules of V. jacquemontii were typical of all other described mimosoid legumes. All 73 of the isolates were strains of Ensifer, and concatenated phylogenetic analysis of their housekeeping genes (rrs, recA, atpD, glnII and dnaK) suggested that they are novel, forming separate lineages close to E. saheli. The phylogenies of the symbiosis-essential genes nodA and nifH were inconsistent with the housekeeping phylogenies. The nodA sequences of most isolates were close to that of E. arboris HAMBI 1552T, but the nifH gene was found to be related to that of E. kostiensis HAMBI 1489T. All the tested Ensifer strains, except for AJ24, were found to be capable of nodulating other species of Vachellia as well as native Indian Mimosa and Prosopis spp. Conclusions: Stressful conditions caused by the alkaline soil of the Thar Desert have resulted in V. jacquemontii being nodulated by diverse and promiscuous Ensifer species that are capable of nodulating other native members of the tribe Mimoseae.",
    keywords = "Ensifer, Multi Locus Sequence Analysis (MLSA), nifH, nodA, rhizobia, Thar Desert, Vachellia (Acacia) jacquemontii",
    author = "Sankhla, {Indu Singh} and Nisha Tak and Meghwal, {Raju Ram} and Sunil Choudhary and Alkesh Tak and Sonam Rathi and Sprent, {Janet I.} and James, {Euan K.} and Gehlot, {Hukam Singh}",
    note = "Indu S. Sankhla thanks the University Grants Commission (UGC), New Delhi, for a junior and senior research fellowship. The work is also supported by grants from the Department of Biotechnology Govt. of India (BT/PR11461/AGR/21/270/2008) and the University Grants Commission (UGC-SAPII-CAS-I, UGC-BSR Start-Up-Grant F.30-16/2014).",
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    Molecular characterization of nitrogen fixing microsymbionts from root nodules of Vachellia (Acacia) jacquemontii, a native legume from the Thar Desert of India. / Sankhla, Indu Singh; Tak, Nisha; Meghwal, Raju Ram; Choudhary, Sunil; Tak, Alkesh; Rathi, Sonam; Sprent, Janet I.; James, Euan K. (Lead / Corresponding author); Gehlot, Hukam Singh (Lead / Corresponding author).

    In: Plant and Soil, Vol. 410, No. 1, 01.2017, p. 21-40.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Molecular characterization of nitrogen fixing microsymbionts from root nodules of Vachellia (Acacia) jacquemontii, a native legume from the Thar Desert of India

    AU - Sankhla, Indu Singh

    AU - Tak, Nisha

    AU - Meghwal, Raju Ram

    AU - Choudhary, Sunil

    AU - Tak, Alkesh

    AU - Rathi, Sonam

    AU - Sprent, Janet I.

    AU - James, Euan K.

    AU - Gehlot, Hukam Singh

    N1 - Indu S. Sankhla thanks the University Grants Commission (UGC), New Delhi, for a junior and senior research fellowship. The work is also supported by grants from the Department of Biotechnology Govt. of India (BT/PR11461/AGR/21/270/2008) and the University Grants Commission (UGC-SAPII-CAS-I, UGC-BSR Start-Up-Grant F.30-16/2014).

    PY - 2017/1

    Y1 - 2017/1

    N2 - Aims: To describe the structure of nodules of Vachellia (Acacia) jacquemontii, and to characterise the rhizobia that occupy them. Methods: Light and electron microscopy were used to analyse nodules. Rhizobia were characterised using their 16S rRNA, housekeeping and symbiosis-related gene sequences. Results: Nodules of V. jacquemontii were typical of all other described mimosoid legumes. All 73 of the isolates were strains of Ensifer, and concatenated phylogenetic analysis of their housekeeping genes (rrs, recA, atpD, glnII and dnaK) suggested that they are novel, forming separate lineages close to E. saheli. The phylogenies of the symbiosis-essential genes nodA and nifH were inconsistent with the housekeeping phylogenies. The nodA sequences of most isolates were close to that of E. arboris HAMBI 1552T, but the nifH gene was found to be related to that of E. kostiensis HAMBI 1489T. All the tested Ensifer strains, except for AJ24, were found to be capable of nodulating other species of Vachellia as well as native Indian Mimosa and Prosopis spp. Conclusions: Stressful conditions caused by the alkaline soil of the Thar Desert have resulted in V. jacquemontii being nodulated by diverse and promiscuous Ensifer species that are capable of nodulating other native members of the tribe Mimoseae.

    AB - Aims: To describe the structure of nodules of Vachellia (Acacia) jacquemontii, and to characterise the rhizobia that occupy them. Methods: Light and electron microscopy were used to analyse nodules. Rhizobia were characterised using their 16S rRNA, housekeeping and symbiosis-related gene sequences. Results: Nodules of V. jacquemontii were typical of all other described mimosoid legumes. All 73 of the isolates were strains of Ensifer, and concatenated phylogenetic analysis of their housekeeping genes (rrs, recA, atpD, glnII and dnaK) suggested that they are novel, forming separate lineages close to E. saheli. The phylogenies of the symbiosis-essential genes nodA and nifH were inconsistent with the housekeeping phylogenies. The nodA sequences of most isolates were close to that of E. arboris HAMBI 1552T, but the nifH gene was found to be related to that of E. kostiensis HAMBI 1489T. All the tested Ensifer strains, except for AJ24, were found to be capable of nodulating other species of Vachellia as well as native Indian Mimosa and Prosopis spp. Conclusions: Stressful conditions caused by the alkaline soil of the Thar Desert have resulted in V. jacquemontii being nodulated by diverse and promiscuous Ensifer species that are capable of nodulating other native members of the tribe Mimoseae.

    KW - Ensifer

    KW - Multi Locus Sequence Analysis (MLSA)

    KW - nifH

    KW - nodA

    KW - rhizobia

    KW - Thar Desert

    KW - Vachellia (Acacia) jacquemontii

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    EP - 40

    JO - Plant and Soil

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