Molecular characterization of the evolution of phagosomes

Jonathan Boulais, Matthias Trost (Lead / Corresponding author), Christian R. Landry, Regis Dieckmann, Emmanuel D. Levy, Thierry Soldati, Stephen W. Michnick, Pierre Thibault (Lead / Corresponding author), Michel Desjardins (Lead / Corresponding author)

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    Abstract

    Amoeba use phagocytosis to internalize bacteria as a source of nutrients, whereas multicellular organisms utilize this process as a defense mechanism to kill microbes and, in vertebrates, initiate a sustained immune response. By using a large-scale approach to identify and compare the proteome and phosphoproteome of phagosomes isolated from distant organisms, and by comparative analysis over 39 taxa, we identified an 'ancient' core of phagosomal proteins around which the immune functions of this organelle have likely organized. Our data indicate that a larger proportion of the phagosome proteome, compared with the whole cell proteome, has been acquired through gene duplication at a period coinciding with the emergence of innate and adaptive immunity. Our study also characterizes in detail the acquisition of novel proteins and the significant remodeling of the phagosome phosphoproteome that contributed to modify the core constituents of this organelle in evolution. Our work thus provides the first thorough analysis of the changes that enabled the transformation of the phagosome from a phagotrophic compartment into an organelle fully competent for antigen presentation. Molecular Systems Biology 6: 423; published online 19 October 2010; doi: 10.1038/msb.2010.80

    Original languageEnglish
    Article number423
    Pages (from-to)-
    Number of pages14
    JournalMolecular Systems Biology
    Volume6
    DOIs
    Publication statusPublished - Oct 2010

    Cite this

    Boulais, J., Trost, M., Landry, C. R., Dieckmann, R., Levy, E. D., Soldati, T., Michnick, S. W., Thibault, P., & Desjardins, M. (2010). Molecular characterization of the evolution of phagosomes. Molecular Systems Biology, 6, -. [423]. https://doi.org/10.1038/msb.2010.80