Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum

Arnaud Javelle; Blanca-Rosa Rodríguez-Pastrana; Christophe Jacob; Bernard Botton; Annick Brun; Bruno André; Anne-Marie Marini; Michel Chalot

doi:10.1016/S0014-5793(01)02802-2

Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum

Arnaud Javelle, Blanca-Rosa Rodríguez-Pastrana, Christophe Jacob, Bernard Botton, Annick Brun, Bruno André, Anne-Marie Marini, Michel Chalot

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Abstract

Heterologous expression of the yeast triple Mep mutant has enabled the first molecular characterization of AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structure of the ectomycorrhizal symbiosis and therefore molecular studies on ammonium transport in hyphae are urgently needed. The kinetic properties of AMT2 and AMT3 from Hebeloma cylindrosporum were studied in Saccharomyces cerevisiae. Expression of HcAmts in the yeast triple Mep mutant restored ammonium retention within cells. The HcAmts did not complement the ammonium sensing defect phenotype of Mep2Delta cells during pseudohyphal differentiation. Northern blot analysis in H. cylindrosporum showed that the HcAMTs were up-regulated upon nitrogen deprivation and down-regulated by ammonium.

Original language	English
Pages (from-to)	393-8
Number of pages	6
Journal	FEBS Letters
Volume	505
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(01)02802-2
Publication status	Published - 2001

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title = "Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum",

abstract = "Heterologous expression of the yeast triple Mep mutant has enabled the first molecular characterization of AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structure of the ectomycorrhizal symbiosis and therefore molecular studies on ammonium transport in hyphae are urgently needed. The kinetic properties of AMT2 and AMT3 from Hebeloma cylindrosporum were studied in Saccharomyces cerevisiae. Expression of HcAmts in the yeast triple Mep mutant restored ammonium retention within cells. The HcAmts did not complement the ammonium sensing defect phenotype of Mep2Delta cells during pseudohyphal differentiation. Northern blot analysis in H. cylindrosporum showed that the HcAMTs were up-regulated upon nitrogen deprivation and down-regulated by ammonium.",

author = "Arnaud Javelle and Blanca-Rosa Rodr{\'i}guez-Pastrana and Christophe Jacob and Bernard Botton and Annick Brun and Bruno Andr{\'e} and Anne-Marie Marini and Michel Chalot",

year = "2001",

doi = "10.1016/S0014-5793(01)02802-2",

language = "English",

volume = "505",

pages = "393--8",

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TY - JOUR

T1 - Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum

AU - Javelle, Arnaud

AU - Rodríguez-Pastrana, Blanca-Rosa

AU - Jacob, Christophe

AU - Botton, Bernard

AU - Brun, Annick

AU - André, Bruno

AU - Marini, Anne-Marie

AU - Chalot, Michel

PY - 2001

Y1 - 2001

N2 - Heterologous expression of the yeast triple Mep mutant has enabled the first molecular characterization of AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structure of the ectomycorrhizal symbiosis and therefore molecular studies on ammonium transport in hyphae are urgently needed. The kinetic properties of AMT2 and AMT3 from Hebeloma cylindrosporum were studied in Saccharomyces cerevisiae. Expression of HcAmts in the yeast triple Mep mutant restored ammonium retention within cells. The HcAmts did not complement the ammonium sensing defect phenotype of Mep2Delta cells during pseudohyphal differentiation. Northern blot analysis in H. cylindrosporum showed that the HcAMTs were up-regulated upon nitrogen deprivation and down-regulated by ammonium.

AB - Heterologous expression of the yeast triple Mep mutant has enabled the first molecular characterization of AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structure of the ectomycorrhizal symbiosis and therefore molecular studies on ammonium transport in hyphae are urgently needed. The kinetic properties of AMT2 and AMT3 from Hebeloma cylindrosporum were studied in Saccharomyces cerevisiae. Expression of HcAmts in the yeast triple Mep mutant restored ammonium retention within cells. The HcAmts did not complement the ammonium sensing defect phenotype of Mep2Delta cells during pseudohyphal differentiation. Northern blot analysis in H. cylindrosporum showed that the HcAMTs were up-regulated upon nitrogen deprivation and down-regulated by ammonium.

U2 - 10.1016/S0014-5793(01)02802-2

DO - 10.1016/S0014-5793(01)02802-2

M3 - Article

C2 - 11576535

SN - 0014-5793

VL - 505

SP - 393

EP - 398

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum

Abstract

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