Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

Yu Hua Chen; Mao Xiang Chen; Dario R. Alessi; David G. Campbell; Catherine Shanahan; Philip Cohen; Patricia T.W. Cohen

doi:10.1016/0014-5793(94)01231-8

Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

Yu Hua Chen, Mao Xiang Chen, Dario R. Alessi, David G. Campbell, Catherine Shanahan, Philip Cohen, Patricia T.W. Cohen

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Abstract

The structures of the M₁₁₀ and M₂₁ regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M₁₁₀ subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M₂₁ subunit from chicken gizzard contains a leucine zipper motif. The M₁₁₀ subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M₂₁ subunit.

Original language	English
Pages (from-to)	51-55
Number of pages	5
Journal	FEBS Letters
Volume	356
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)01231-8
Publication status	Published - 14 Dec 1994

Keywords

Ankyrin repeat
cDNA sequence
Leucine zipper
Myosin
Protein phosphatase
Smooth muscle

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M",

abstract = "The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.",

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AU - Chen, Yu Hua

AU - Chen, Mao Xiang

AU - Alessi, Dario R.

AU - Campbell, David G.

AU - Shanahan, Catherine

AU - Cohen, Philip

AU - Cohen, Patricia T.W.

PY - 1994/12/14

Y1 - 1994/12/14

N2 - The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.

AB - The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.

KW - Ankyrin repeat

KW - cDNA sequence

KW - Leucine zipper

KW - Myosin

KW - Protein phosphatase

KW - Smooth muscle

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Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

Abstract

Keywords

ASJC Scopus subject areas

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