Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

Yu Hua Chen, Mao Xiang Chen, Dario R. Alessi, David G. Campbell, Catherine Shanahan, Philip Cohen, Patricia T.W. Cohen

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)

Abstract

The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalFEBS Letters
Volume356
Issue number1
DOIs
Publication statusPublished - 14 Dec 1994

Keywords

  • Ankyrin repeat
  • cDNA sequence
  • Leucine zipper
  • Myosin
  • Protein phosphatase
  • Smooth muscle

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