Molecular determinants for nuclear import of influenza A PB2 by importin α isoforms 3 and 7

Ruth A. Pumroy, Song Ke, Darren Hart, Ulrich Zachariae, Gino Cingolani (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)


Influenza A virus polymerase subunit PB2 is a major virulence determinant implicated in pathogenicity and host adaptation. During cross-species virus transfer from avian to mammalian cells, PB2 switches specificity from importin α3 to α7. This specificity is not recapitulated invitro, where PB2 binds all importin α isoforms with comparably high affinity. In this study, we investigated the structure, conformational dynamics, and autoinhibition of importin α isoforms 1, 3, and 7 in complex with PB2. Our data suggest that association of PB2 with α3 and α7 is favored by reduced autoinhibition of these isoforms and by the unique structure of the nuclear localization signal (NLS) domain of PB2. We propose that by recruiting importin α3 or α7 in the absence of importin β, PB2 reduces the complexity of adaptor-mediated import to a pseudo-bimolecular reaction, thereby acquiring a kinetic advantage over classical NLS cargos, which form an import complex only when importin α and β are simultaneously available. The human genome encodes seven isoforms of importin α, grouped into three subfamilies. Pumroy etal. show that recruitment of the influenza A virus PB2 subunit by importin α3 and α7 is favored by reduced IBB autoinhibition and, in the case of importin α3, enhanced conformational flexibility of the Arm core.
Original languageEnglish
Pages (from-to)374-384
Number of pages11
Issue number2
Early online date15 Jan 2015
Publication statusPublished - 3 Feb 2015


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