Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex

A. Graner; S. Streng; A. Kellermann; A. Schiemann; E. Bauer; R. Waugh; B. Pellio; F. Ordon

doi:10.1007/s001220051070

Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex

A. Graner, S. Streng, A. Kellermann, A. Schiemann, E. Bauer, R. Waugh, B. Pellio, F. Ordon

Plant Sciences

Research output: Contribution to journal › Article › peer-review

84 Citations (Scopus)

Abstract

The genetic structure of the rym5 locus was studied in a population comprising 391 doubled-haploid lines that were evaluated for resistance to two strains of Barley Yellow Mosaic Virus (BaYMV-1, 2) and to Barley Mild Mosaic Virus (BaMMV). The absence of recombinants that are able to differentiate between the reaction to these different bymoviruses provides evidence that rym5 is a complex locus, which is either composed of several closely linked genes or of an allelic series of a single gene. For marker-assisted introgression of this locus into adapted barley germplasm, a CAPS (cleaved amplified polymorphic sequence) and a microsatellite marker were developed that flank the gene at distances of 0.8 and 1.3% recombination, respectively.

Original language	English
Pages (from-to)	285-290
Number of pages	6
Journal	Theoretical and Applied Genetics
Volume	98
Issue number	2
DOIs	https://doi.org/10.1007/s001220051070
Publication status	Published - 1 Jan 1999

Keywords

Disease resistance
Hordeum vulgate
Marker-assisted selection
Molecular markers
PCR

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Genetics

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10.1007/s001220051070

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title = "Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex",

abstract = "The genetic structure of the rym5 locus was studied in a population comprising 391 doubled-haploid lines that were evaluated for resistance to two strains of Barley Yellow Mosaic Virus (BaYMV-1, 2) and to Barley Mild Mosaic Virus (BaMMV). The absence of recombinants that are able to differentiate between the reaction to these different bymoviruses provides evidence that rym5 is a complex locus, which is either composed of several closely linked genes or of an allelic series of a single gene. For marker-assisted introgression of this locus into adapted barley germplasm, a CAPS (cleaved amplified polymorphic sequence) and a microsatellite marker were developed that flank the gene at distances of 0.8 and 1.3% recombination, respectively.",

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AU - Graner, A.

AU - Streng, S.

AU - Kellermann, A.

AU - Schiemann, A.

AU - Bauer, E.

AU - Waugh, R.

AU - Pellio, B.

AU - Ordon, F.

PY - 1999/1/1

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N2 - The genetic structure of the rym5 locus was studied in a population comprising 391 doubled-haploid lines that were evaluated for resistance to two strains of Barley Yellow Mosaic Virus (BaYMV-1, 2) and to Barley Mild Mosaic Virus (BaMMV). The absence of recombinants that are able to differentiate between the reaction to these different bymoviruses provides evidence that rym5 is a complex locus, which is either composed of several closely linked genes or of an allelic series of a single gene. For marker-assisted introgression of this locus into adapted barley germplasm, a CAPS (cleaved amplified polymorphic sequence) and a microsatellite marker were developed that flank the gene at distances of 0.8 and 1.3% recombination, respectively.

AB - The genetic structure of the rym5 locus was studied in a population comprising 391 doubled-haploid lines that were evaluated for resistance to two strains of Barley Yellow Mosaic Virus (BaYMV-1, 2) and to Barley Mild Mosaic Virus (BaMMV). The absence of recombinants that are able to differentiate between the reaction to these different bymoviruses provides evidence that rym5 is a complex locus, which is either composed of several closely linked genes or of an allelic series of a single gene. For marker-assisted introgression of this locus into adapted barley germplasm, a CAPS (cleaved amplified polymorphic sequence) and a microsatellite marker were developed that flank the gene at distances of 0.8 and 1.3% recombination, respectively.

KW - Disease resistance

KW - Hordeum vulgate

KW - Marker-assisted selection

KW - Molecular markers

KW - PCR

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Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex

Abstract

Keywords

ASJC Scopus subject areas

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