A combination of random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers has been used to locate genes controlling important developmental characters in barley. The denso dwarfing gene has been mapped to the long arm of chromosome 3H. Stepwise multiple regression was also used to identify another region of the barley genome (on chromosome 7H), which contributed to variation in height. The denso locus was shown to be associated with delaying time to heading. A protein (WSP2) and an RAPD marker on barley chromosomes 5H and 6H, respectively, were also associated with time to heading. These results are discussed in relation to the genetic analysis of developmentally important traits and the development of dwarfing genes in barley breeding programs.