Monitoring alternative splicing changes in Arabidopsis circadian clock genes

Craig G. Simpson; John Fuller; Cristiane P. G. Calixto; Jim McNicol; Clare Booth; John W. S. Brown; Dorothee Staiger

doi:10.1007/978-1-4939-3356-3_11

Monitoring alternative splicing changes in Arabidopsis circadian clock genes

Craig G. Simpson (Lead / Corresponding author), John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, Dorothee Staiger

Plant Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

10 Citations (Scopus)

Abstract

Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments.

Original language	English
Title of host publication	Environmental responses in plants
Subtitle of host publication	methods and protocols
Editors	Paula Duque
Place of Publication	New York
Publisher	Springer
Pages	119-132
Number of pages	14
ISBN (Electronic)	9781493933563
ISBN (Print)	9781493933549
DOIs	https://doi.org/10.1007/978-1-4939-3356-3_11
Publication status	Published - 2016

Publication series

Name	Methods in molecular biology
Publisher	Springer
Volume	1398
ISSN (Print)	1064-3745

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10.1007/978-1-4939-3356-3_11

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Simpson, C. G., Fuller, J., Calixto, C. P. G., McNicol, J., Booth, C., Brown, J. W. S., & Staiger, D. (2016). Monitoring alternative splicing changes in Arabidopsis circadian clock genes. In P. Duque (Ed.), Environmental responses in plants: methods and protocols (pp. 119-132). (Methods in molecular biology; Vol. 1398). Springer . https://doi.org/10.1007/978-1-4939-3356-3_11

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Monitoring alternative splicing changes in Arabidopsis circadian clock genes. / Simpson, Craig G. (Lead / Corresponding author); Fuller, John; Calixto, Cristiane P. G. et al.
Environmental responses in plants: methods and protocols. ed. / Paula Duque. New York: Springer , 2016. p. 119-132 (Methods in molecular biology; Vol. 1398).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Monitoring alternative splicing changes in Arabidopsis circadian clock genes

AU - Simpson, Craig G.

AU - Fuller, John

AU - Calixto, Cristiane P. G.

AU - McNicol, Jim

AU - Booth, Clare

AU - Brown, John W. S.

AU - Staiger, Dorothee

PY - 2016

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N2 - Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments.

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DO - 10.1007/978-1-4939-3356-3_11

M3 - Chapter (peer-reviewed)

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T3 - Methods in molecular biology

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EP - 132

BT - Environmental responses in plants

A2 - Duque, Paula

PB - Springer

CY - New York

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Monitoring alternative splicing changes in Arabidopsis circadian clock genes

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