Monitoring alternative splicing changes in Arabidopsis circadian clock genes

Craig G. Simpson (Lead / Corresponding author), John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, Dorothee Staiger

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

7 Citations (Scopus)

Abstract

Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments.

Original languageEnglish
Title of host publicationEnvironmental responses in plants
Subtitle of host publicationmethods and protocols
EditorsPaula Duque
Place of PublicationNew York
PublisherSpringer
Pages119-132
Number of pages14
ISBN (Electronic)9781493933563
ISBN (Print)9781493933549
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume1398
ISSN (Print)1064-3745

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  • Cite this

    Simpson, C. G., Fuller, J., Calixto, C. P. G., McNicol, J., Booth, C., Brown, J. W. S., & Staiger, D. (2016). Monitoring alternative splicing changes in Arabidopsis circadian clock genes. In P. Duque (Ed.), Environmental responses in plants: methods and protocols (pp. 119-132). (Methods in molecular biology; Vol. 1398). Springer . https://doi.org/10.1007/978-1-4939-3356-3_11