Morphological and genetic characterisation of the root system architecture of selected barley recombinant chromosome substitution lines using an integrated phenotyping approach

C. De La Fuente Canto, D. I. Kalogiros, M. Ptashnyk, T. S. George, R. Waugh, A. G. Bengough, J. Russell, L. X. Dupuy

Research output: Contribution to journalArticle

2 Citations (Scopus)
104 Downloads (Pure)

Abstract

Discoveries on the genetics of resource acquisition efficiency are limited by the ability to measure plant roots in sufficient number and with adequate genotypic variability. This paper presents a root phenotyping study that explores ways to combine live imaging and computer algorithms for model-based extraction of root growth parameters. The study is based on a subset of barley Recombinant Chromosome Substitution Lines (RCSLs) and a combinatorial approach was designed for fast identification of the regions of the genome that contribute the most to variations in root system architecture (RSA). Results showed there was a strong genotypic variation in root growth parameters within the set of genotypes studied. The chromosomal regions associated with primary root growth differed from the regions of the genome associated with changes in lateral root growth. The concepts presented here are discussed in the context of identifying root QTL and its potential to assist breeding for novel crops with improved root systems.

Original languageEnglish
Pages (from-to)84-97
Number of pages14
JournalJournal of Theoretical Biology
Volume447
Early online date17 Mar 2018
DOIs
Publication statusPublished - 14 Jun 2018

Fingerprint

Root Growth
substitution lines
Barley
Root System
Hordeum
Chromosomes
System Architecture
Chromosome
root systems
Substitution
root growth
Substitution reactions
barley
phenotype
Line
Roots
Growth
Genome
Genes
Quantitative Trait Loci

Keywords

  • Journal Article
  • rootphenotyping
  • QTL
  • barley
  • RCSL
  • growth parameters
  • computer assisted breeding

Cite this

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title = "Morphological and genetic characterisation of the root system architecture of selected barley recombinant chromosome substitution lines using an integrated phenotyping approach",
abstract = "Discoveries on the genetics of resource acquisition efficiency are limited by the ability to measure plant roots in sufficient number and with adequate genotypic variability. This paper presents a root phenotyping study that explores ways to combine live imaging and computer algorithms for model-based extraction of root growth parameters. The study is based on a subset of barley Recombinant Chromosome Substitution Lines (RCSLs) and a combinatorial approach was designed for fast identification of the regions of the genome that contribute the most to variations in root system architecture (RSA). Results showed there was a strong genotypic variation in root growth parameters within the set of genotypes studied. The chromosomal regions associated with primary root growth differed from the regions of the genome associated with changes in lateral root growth. The concepts presented here are discussed in the context of identifying root QTL and its potential to assist breeding for novel crops with improved root systems.",
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note = "Michael Adu provided invaluable help during the development of the experimental set up. We also thank Prof. X. Draye for useful discussions during the design of this work and Philip White for the valuable comments provided on the manuscript. This work was supported by the EU FP7 project EURoot ‘Enhanced models for predicting RSA development under multiple stresses’ (2011–2016, KBBE.2011.1.2-05 no. 289300). The James Hutton Institute received support from the Scottish Government Rural and Environment Science and Analytical Services Division (RESAS, Workpackage 2.1.7, 2.3.4)",
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Morphological and genetic characterisation of the root system architecture of selected barley recombinant chromosome substitution lines using an integrated phenotyping approach. / Canto, C. De La Fuente; Kalogiros, D. I.; Ptashnyk, M.; George, T. S.; Waugh, R.; Bengough, A. G.; Russell, J.; Dupuy, L. X.

In: Journal of Theoretical Biology, Vol. 447, 14.06.2018, p. 84-97.

Research output: Contribution to journalArticle

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AU - Canto, C. De La Fuente

AU - Kalogiros, D. I.

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AU - George, T. S.

AU - Waugh, R.

AU - Bengough, A. G.

AU - Russell, J.

AU - Dupuy, L. X.

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