Morphological evidence that A-CAM is a major intercellular adhesion molecule in human kidney

L. R. Biddlestone, S. Fleming

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    19 Citations (Scopus)

    Abstract

    We have used immunocytochemistry to identify the major primary adhesion molecule of the cadherin class in human kidney. In frozen sections of kidney, A-CAM was detected using the monoclonal antibody GC4 on the surface of renal tubular epithelial cells. Renal tubular epithelium did not express L-CAM. No cadherin reactivity was found on the glomerular epithelial cells. Cultured renal tubular epithelium was studied by immunofluorescence and immunogold methods. A-CAM was found at the contact points of adjacent epithelial cells, the phenotype of which was confirmed by the demonstration of cytokeratins using the antibody CAM 5.2. The A-CAM molecule in human kidney had an Mr of 130 kD in Western blotting experiments. These results lead us to conclude that A-CAM is the major cadherin of adult human renal epithelium.

    Original languageEnglish
    Pages (from-to)9-15
    Number of pages7
    JournalJournal of Pathology
    Volume164
    Issue number1
    DOIs
    Publication statusPublished - 1991

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