Morphological evidence that A-CAM is a major intercellular adhesion molecule in human kidney

TY - JOUR

T1 - Morphological evidence that A-CAM is a major intercellular adhesion molecule in human kidney

AU - Biddlestone, L. R.

AU - Fleming, S.

PY - 1991

Y1 - 1991

N2 - We have used immunocytochemistry to identify the major primary adhesion molecule of the cadherin class in human kidney. In frozen sections of kidney, A-CAM was detected using the monoclonal antibody GC4 on the surface of renal tubular epithelial cells. Renal tubular epithelium did not express L-CAM. No cadherin reactivity was found on the glomerular epithelial cells. Cultured renal tubular epithelium was studied by immunofluorescence and immunogold methods. A-CAM was found at the contact points of adjacent epithelial cells, the phenotype of which was confirmed by the demonstration of cytokeratins using the antibody CAM 5.2. The A-CAM molecule in human kidney had an Mr of 130 kD in Western blotting experiments. These results lead us to conclude that A-CAM is the major cadherin of adult human renal epithelium.

AB - We have used immunocytochemistry to identify the major primary adhesion molecule of the cadherin class in human kidney. In frozen sections of kidney, A-CAM was detected using the monoclonal antibody GC4 on the surface of renal tubular epithelial cells. Renal tubular epithelium did not express L-CAM. No cadherin reactivity was found on the glomerular epithelial cells. Cultured renal tubular epithelium was studied by immunofluorescence and immunogold methods. A-CAM was found at the contact points of adjacent epithelial cells, the phenotype of which was confirmed by the demonstration of cytokeratins using the antibody CAM 5.2. The A-CAM molecule in human kidney had an Mr of 130 kD in Western blotting experiments. These results lead us to conclude that A-CAM is the major cadherin of adult human renal epithelium.

U2 - 10.1002/path.1711640103

DO - 10.1002/path.1711640103

M3 - Article

C2 - 2056393

SN - 0022-3417

VL - 164

SP - 9

EP - 15

JO - Journal of Pathology

JF - Journal of Pathology

IS - 1

ER -