Mps1 promotes rapid centromere accumulation of Aurora B

Maike S. van der Waal, Adrian T. Saurin, Martijn J. M. Vromans, Mathijs Vleugel, Claudia Wurzenberger, Daniel W. Gerlich, Rene H. Medema, Geert J. P. L. Kops, Susanne M. A. Lens

    Research output: Contribution to journalArticlepeer-review

    69 Citations (Scopus)

    Abstract

    Aurora B localization to mitotic centromeres, which is required for proper chromosome alignment during mitosis, relies on Haspin-dependent histone H3 phosphorylation and on Bub1-dependent histone H2A phosphorylation-which interacts with Borealin through a Shugoshin (Sgo) intermediate. We demonstrate that Mps1 stimulates the latter recruitment axis. Mps1 activity enhances H2A-T120ph and is critical for Sgo1 recruitment to centromeres, thereby promoting Aurora B centromere recruitment in early mitosis. Importantly, chromosome biorientation defects caused by Mps1 inhibition are improved by restoring Aurora B centromere recruitment. As Mps1 kinetochore localization reciprocally depends on Aurora B, we propose that this Aurora B-Mps1 recruitment circuitry cooperates with the Aurora B-Haspin feedback loop to ensure rapid centromere accumulation of Aurora B at the onset of mitosis.

    Original languageEnglish
    Pages (from-to)847-854
    Number of pages8
    JournalEMBO Reports
    Volume13
    Issue number9
    DOIs
    Publication statusPublished - Sept 2012

    Keywords

    • SPINDLE ASSEMBLY CHECKPOINT
    • ORIENTATION
    • CHROMOSOME BIORIENTATION
    • PHOSPHORYLATION GRADIENT
    • ALIGNMENT
    • ANAPHASE
    • biorientation
    • centromere
    • Aurora B
    • ACTIVATION
    • KINETOCHORES
    • Mps1
    • KINASE
    • MITOSIS

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