Mps1 promotes rapid centromere accumulation of Aurora B

Maike S. van der Waal; Adrian T. Saurin; Martijn J. M. Vromans; Mathijs Vleugel; Claudia Wurzenberger; Daniel W. Gerlich; Rene H. Medema; Geert J. P. L. Kops; Susanne M. A. Lens

doi:10.1038/embor.2012.93

Mps1 promotes rapid centromere accumulation of Aurora B

Maike S. van der Waal
, Adrian T. Saurin
, Martijn J. M. Vromans
, Mathijs Vleugel
, Claudia Wurzenberger
, Daniel W. Gerlich
, Rene H. Medema
, Geert J. P. L. Kops
, Susanne M. A. Lens

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

Aurora B localization to mitotic centromeres, which is required for proper chromosome alignment during mitosis, relies on Haspin-dependent histone H3 phosphorylation and on Bub1-dependent histone H2A phosphorylation-which interacts with Borealin through a Shugoshin (Sgo) intermediate. We demonstrate that Mps1 stimulates the latter recruitment axis. Mps1 activity enhances H2A-T120ph and is critical for Sgo1 recruitment to centromeres, thereby promoting Aurora B centromere recruitment in early mitosis. Importantly, chromosome biorientation defects caused by Mps1 inhibition are improved by restoring Aurora B centromere recruitment. As Mps1 kinetochore localization reciprocally depends on Aurora B, we propose that this Aurora B-Mps1 recruitment circuitry cooperates with the Aurora B-Haspin feedback loop to ensure rapid centromere accumulation of Aurora B at the onset of mitosis.

Original language	English
Pages (from-to)	847-854
Number of pages	8
Journal	EMBO Reports
Volume	13
Issue number	9
DOIs	https://doi.org/10.1038/embor.2012.93
Publication status	Published - Sept 2012

Keywords

SPINDLE ASSEMBLY CHECKPOINT
ORIENTATION
CHROMOSOME BIORIENTATION
PHOSPHORYLATION GRADIENT
ALIGNMENT
ANAPHASE
biorientation
centromere
Aurora B
ACTIVATION
KINETOCHORES
Mps1
KINASE
MITOSIS

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@article{9549d26ec71540198389cfe8c4d37d35,

title = "Mps1 promotes rapid centromere accumulation of Aurora B",

abstract = "Aurora B localization to mitotic centromeres, which is required for proper chromosome alignment during mitosis, relies on Haspin-dependent histone H3 phosphorylation and on Bub1-dependent histone H2A phosphorylation-which interacts with Borealin through a Shugoshin (Sgo) intermediate. We demonstrate that Mps1 stimulates the latter recruitment axis. Mps1 activity enhances H2A-T120ph and is critical for Sgo1 recruitment to centromeres, thereby promoting Aurora B centromere recruitment in early mitosis. Importantly, chromosome biorientation defects caused by Mps1 inhibition are improved by restoring Aurora B centromere recruitment. As Mps1 kinetochore localization reciprocally depends on Aurora B, we propose that this Aurora B-Mps1 recruitment circuitry cooperates with the Aurora B-Haspin feedback loop to ensure rapid centromere accumulation of Aurora B at the onset of mitosis.",

keywords = "SPINDLE ASSEMBLY CHECKPOINT, ORIENTATION, CHROMOSOME BIORIENTATION, PHOSPHORYLATION GRADIENT, ALIGNMENT, ANAPHASE, biorientation, centromere, Aurora B, ACTIVATION, KINETOCHORES, Mps1, KINASE, MITOSIS",

author = "\{van der Waal\}, \{Maike S.\} and Saurin, \{Adrian T.\} and Vromans, \{Martijn J. M.\} and Mathijs Vleugel and Claudia Wurzenberger and Gerlich, \{Daniel W.\} and Medema, \{Rene H.\} and Kops, \{Geert J. P. L.\} and Lens, \{Susanne M. A.\}",

year = "2012",

month = sep,

doi = "10.1038/embor.2012.93",

language = "English",

volume = "13",

pages = "847--854",

journal = "EMBO Reports",

issn = "1469-221X",

publisher = "Springer Science and Business Media Deutschland GmbH",

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T1 - Mps1 promotes rapid centromere accumulation of Aurora B

AU - van der Waal, Maike S.

AU - Saurin, Adrian T.

AU - Vromans, Martijn J. M.

AU - Vleugel, Mathijs

AU - Wurzenberger, Claudia

AU - Gerlich, Daniel W.

AU - Medema, Rene H.

AU - Kops, Geert J. P. L.

AU - Lens, Susanne M. A.

PY - 2012/9

Y1 - 2012/9

N2 - Aurora B localization to mitotic centromeres, which is required for proper chromosome alignment during mitosis, relies on Haspin-dependent histone H3 phosphorylation and on Bub1-dependent histone H2A phosphorylation-which interacts with Borealin through a Shugoshin (Sgo) intermediate. We demonstrate that Mps1 stimulates the latter recruitment axis. Mps1 activity enhances H2A-T120ph and is critical for Sgo1 recruitment to centromeres, thereby promoting Aurora B centromere recruitment in early mitosis. Importantly, chromosome biorientation defects caused by Mps1 inhibition are improved by restoring Aurora B centromere recruitment. As Mps1 kinetochore localization reciprocally depends on Aurora B, we propose that this Aurora B-Mps1 recruitment circuitry cooperates with the Aurora B-Haspin feedback loop to ensure rapid centromere accumulation of Aurora B at the onset of mitosis.

AB - Aurora B localization to mitotic centromeres, which is required for proper chromosome alignment during mitosis, relies on Haspin-dependent histone H3 phosphorylation and on Bub1-dependent histone H2A phosphorylation-which interacts with Borealin through a Shugoshin (Sgo) intermediate. We demonstrate that Mps1 stimulates the latter recruitment axis. Mps1 activity enhances H2A-T120ph and is critical for Sgo1 recruitment to centromeres, thereby promoting Aurora B centromere recruitment in early mitosis. Importantly, chromosome biorientation defects caused by Mps1 inhibition are improved by restoring Aurora B centromere recruitment. As Mps1 kinetochore localization reciprocally depends on Aurora B, we propose that this Aurora B-Mps1 recruitment circuitry cooperates with the Aurora B-Haspin feedback loop to ensure rapid centromere accumulation of Aurora B at the onset of mitosis.

KW - SPINDLE ASSEMBLY CHECKPOINT

KW - ORIENTATION

KW - CHROMOSOME BIORIENTATION

KW - PHOSPHORYLATION GRADIENT

KW - ALIGNMENT

KW - ANAPHASE

KW - biorientation

KW - centromere

KW - Aurora B

KW - ACTIVATION

KW - KINETOCHORES

KW - Mps1

KW - KINASE

KW - MITOSIS

U2 - 10.1038/embor.2012.93

DO - 10.1038/embor.2012.93

M3 - Article

SN - 1469-221X

VL - 13

SP - 847

EP - 854

JO - EMBO Reports

JF - EMBO Reports

IS - 9

ER -

Mps1 promotes rapid centromere accumulation of Aurora B

Abstract

Keywords

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