Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53

Helene Tourriere, Gwennaëlle Versini, Violeta Cordón-Preciado, Constance Alabert, Philippe Pasero (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

209 Citations (Scopus)

Abstract

The yeast checkpoint factors Mrc1p and Tof1p travel with the replication fork and mediate the activation of the Rad53p kinase in response to a replication stress. We show here that both proteins are required for normal fork progression but play different roles at stalled forks. Tof1p is critical for the activity of the rDNA replication fork barrier (RFB) but plays a minor role in the replication checkpoint. In contrast, Mrc1p is not necessary for RFB activity but is essential to mediate the replication stress response. Interestingly, stalled forks did not collapse in mrc1Δ cells exposed to hydroxyurea (HU) as they do in rad53 mutants. However, forks failed to restart when mrc1Δ cells were released from the block. The critical role of Mrc1p in HU is therefore to promote fork recovery in a Rad53p-independent manner, presumably through the formation of a stable fork-pausing complex.
Original languageEnglish
Pages (from-to)699-706
Number of pages8
JournalMolecular Cell
Volume19
Issue number5
DOIs
Publication statusPublished - 2 Sept 2005

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