MSK1 and MSK2 are required for the mitogen- and stress-induced phosphorylation of CREB and ATF1 in fibroblasts

Giselle R. Wiggin, Ana Soloaga, Julia M. Foster, Victoria Murray-Tait, Philip Cohen, J. Simon C. Arthur

    Research output: Contribution to journalArticle

    331 Citations (Scopus)

    Abstract

    Using mouse knockouts for mitogen- and stress-activated protein kinase 1 (MSK1) and MSK2 and a double knockout of both MSK1 and MSK2, we show that these protein kinases are required for the stress-induced phosphorylation of transcription factors CREB and ATF1 in primary embryonic fibroblasts. In contrast mitogen-induced phosphorylation of CREB and ATF1 is greatly reduced but not totally abolished. The mitogen- and stress-induced phosphorylation of CREB at Ser133 has been linked to the transcription of several immediate early genes, including c-fos, junB, and egr1. The knockout of both MSK1 and MSK2 resulted in a 50% reduction in c-fos and junB gene transcription in response to anisomycin or UV-C radiation but only a small reduction in response to tetradecanoyl phorbol acetate or epidermal growth factor in fibroblasts. The transcription of egr1 in response to both mitogenic and stress stimuli, as well as stress-induced apoptosis, was unaffected in the MSK1/MSK2 double knockout.
    Original languageEnglish
    Pages (from-to)2871-2881
    Number of pages11
    JournalMolecular and Cellular Biology
    Volume22
    Issue number8
    DOIs
    Publication statusPublished - Apr 2002

    Keywords

    • MSK

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