MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells

    Research output: Contribution to journalArticle

    154 Citations (Scopus)

    Abstract

    Mouse embryonic stem (ES) cells homozygous for disruption of the MSK1 gene had no detectable MSK1 activity. However, their activators (extracellular signal related kinase (ERK)1/ERK2) were stimulated normally in mitogen- and stress-activated protein kinase (MSK)1-/- and wild type cells in response to tetradecanoylphorbol acetate (TPA) and epidermal growth factor (EGF). TPA and EGF induced the phosphorylation of cyclic AMP-responsive element binding protein (CREB) at Ser-133 and ATF1 at Ser-63 in wild type cells and this was abolished by inhibition of the mitogen-activated protein kinase cascade. In contrast, the TPA- and EGF-induced phosphorylation of CREB/ATF1 was barely detectable in MSK1-/- cells. However, basal and forskolin-induced phosphorylation was similar, indicating that the MSK1 'knockout' did not prevent CREB phosphorylation by cyclic AMP-dependent protein kinase. Thus MSK1 is required for CREB and ATF1 phosphorylation after mitogenic stimulation of ES cells.
    Original languageEnglish
    Pages (from-to)44-48
    Number of pages5
    JournalFEBS Letters
    Volume482
    Issue number1-2
    DOIs
    Publication statusPublished - 29 Sep 2000

    Fingerprint

    Phosphorylation
    Stem cells
    Mitogens
    Cyclic AMP
    Carrier Proteins
    Tetradecanoylphorbol Acetate
    Epidermal Growth Factor
    Colforsin
    Embryonic Stem Cells
    Cyclic AMP-Dependent Protein Kinases
    Mitogen-Activated Protein Kinases
    Phosphotransferases
    Genes
    Mouse Embryonic Stem Cells

    Keywords

    • Animals
    • Tetradecanoylphorbol Acetate
    • Calcium-Calmodulin-Dependent Protein Kinases
    • Recombinant Proteins
    • Epidermal Growth Factor
    • Chromosomes, Artificial, Bacterial
    • Mice
    • Cyclic AMP Response Element-Binding Protein
    • Cloning, Molecular
    • Mice, Knockout
    • Mice, Inbred Strains
    • Phosphorylation
    • Ribosomal Protein S6 Kinases, 90-kDa
    • Restriction Mapping
    • Acetyltransferases
    • Proteins
    • Mitogen-Activated Protein Kinase 3
    • Mitogen-Activated Protein Kinases
    • Mitogen-Activated Protein Kinase 1
    • Stem Cells
    • Embryo, Mammalian

    Cite this

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    title = "MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells",
    abstract = "Mouse embryonic stem (ES) cells homozygous for disruption of the MSK1 gene had no detectable MSK1 activity. However, their activators (extracellular signal related kinase (ERK)1/ERK2) were stimulated normally in mitogen- and stress-activated protein kinase (MSK)1-/- and wild type cells in response to tetradecanoylphorbol acetate (TPA) and epidermal growth factor (EGF). TPA and EGF induced the phosphorylation of cyclic AMP-responsive element binding protein (CREB) at Ser-133 and ATF1 at Ser-63 in wild type cells and this was abolished by inhibition of the mitogen-activated protein kinase cascade. In contrast, the TPA- and EGF-induced phosphorylation of CREB/ATF1 was barely detectable in MSK1-/- cells. However, basal and forskolin-induced phosphorylation was similar, indicating that the MSK1 'knockout' did not prevent CREB phosphorylation by cyclic AMP-dependent protein kinase. Thus MSK1 is required for CREB and ATF1 phosphorylation after mitogenic stimulation of ES cells.",
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    author = "Arthur, {J. Simson C.} and Philip Cohen",
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    language = "English",
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    MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells. / Arthur, J. Simson C.; Cohen, Philip.

    In: FEBS Letters, Vol. 482, No. 1-2, 29.09.2000, p. 44-48.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells

    AU - Arthur, J. Simson C.

    AU - Cohen, Philip

    PY - 2000/9/29

    Y1 - 2000/9/29

    N2 - Mouse embryonic stem (ES) cells homozygous for disruption of the MSK1 gene had no detectable MSK1 activity. However, their activators (extracellular signal related kinase (ERK)1/ERK2) were stimulated normally in mitogen- and stress-activated protein kinase (MSK)1-/- and wild type cells in response to tetradecanoylphorbol acetate (TPA) and epidermal growth factor (EGF). TPA and EGF induced the phosphorylation of cyclic AMP-responsive element binding protein (CREB) at Ser-133 and ATF1 at Ser-63 in wild type cells and this was abolished by inhibition of the mitogen-activated protein kinase cascade. In contrast, the TPA- and EGF-induced phosphorylation of CREB/ATF1 was barely detectable in MSK1-/- cells. However, basal and forskolin-induced phosphorylation was similar, indicating that the MSK1 'knockout' did not prevent CREB phosphorylation by cyclic AMP-dependent protein kinase. Thus MSK1 is required for CREB and ATF1 phosphorylation after mitogenic stimulation of ES cells.

    AB - Mouse embryonic stem (ES) cells homozygous for disruption of the MSK1 gene had no detectable MSK1 activity. However, their activators (extracellular signal related kinase (ERK)1/ERK2) were stimulated normally in mitogen- and stress-activated protein kinase (MSK)1-/- and wild type cells in response to tetradecanoylphorbol acetate (TPA) and epidermal growth factor (EGF). TPA and EGF induced the phosphorylation of cyclic AMP-responsive element binding protein (CREB) at Ser-133 and ATF1 at Ser-63 in wild type cells and this was abolished by inhibition of the mitogen-activated protein kinase cascade. In contrast, the TPA- and EGF-induced phosphorylation of CREB/ATF1 was barely detectable in MSK1-/- cells. However, basal and forskolin-induced phosphorylation was similar, indicating that the MSK1 'knockout' did not prevent CREB phosphorylation by cyclic AMP-dependent protein kinase. Thus MSK1 is required for CREB and ATF1 phosphorylation after mitogenic stimulation of ES cells.

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