Multi locus sequence analysis and symbiotic characterization of novel Ensifer strains nodulating Tephrosia spp. in the Indian Thar Desert

Nisha Tak (Lead / Corresponding author), Esha Awasthi, Garima Bissa, Raju Ram Meghwal, Euan K. James, Janet S. Sprent, Hukam S. Gehlot (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Phylogenetically diverse Ensifer strains associated with five species of Tephrosia growing in alkaline soils of semi-arid regions of the Thar Desert were characterized using multi locus sequence analysis. Based on 16S rRNA and four protein-coding housekeeping gene (recA, atpD, glnII and dnaK) sequences, the Tephrosia-Ensifer strains were genetically different from the type strains of Ensifer saheli, Ensifer kostiensis, Ensifer terangae (African origin) and Ensifer psoraleae (Asiatic origin). One strain, Ensifer sp. TL4, showed maximum similarity (99%) to Ensifer adhaerens LMG 20216(T) and formed a separate lineage close to it. Phylogenetic incongruence between sym and housekeeping genes was observed. The monophyletic origin of symbiotic genes from Asia in the Tephrosia-Ensifer strains from the Thar Desert suggests that they might have been acquired from a common ancestor and horizontally transferred. These novel strains are promiscuous, cross-nodulating some papilionoid crop species, mimosoid trees and the caesalpinioid Chamaecrista pumila. This study improves understanding of the distribution of Ensifer in unexplored and threatened alkaline arid regions of the Thar Desert and how this relates to other similar regions in the world.

Original languageEnglish
Pages (from-to)534-545
Number of pages12
JournalSystematic and Applied Microbiology
Volume39
Issue number8
Early online date4 Oct 2016
DOIs
Publication statusPublished - Dec 2016

Keywords

  • Ensifer
  • Multi locus sequence analysis (MLSA)
  • Tephrosia
  • Symbiosis
  • Thar Desert
  • Legume

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