Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors

Marine J. Petit; Charlotte Flory; Quan Gu; Mazigh Fares; Douglas Lamont; Alan Score; Kelsey Davies; Lesley Bell-Sakyi; Pietro Scaturro; Benjamin Brennan; Alain Kohl

doi:10.1038/s41467-025-59565-w

Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors

Marine J. Petit
, Charlotte Flory
, Quan Gu
, Mazigh Fares
, Douglas Lamont
, Alan Score
, Kelsey Davies
, Lesley Bell-Sakyi
, Pietro Scaturro
, Benjamin Brennan (Lead / Corresponding author)
, Alain Kohl (Lead / Corresponding author)

Centre for Advanced Scientific Technologies

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

53 Downloads (Pure)

Abstract

The increasing prevalence of tick-borne arboviral infections worldwide necessitates advanced control strategies, particularly those targeting vectors, to mitigate the disease burden. However, the cellular interactions between arboviruses and ticks, especially for negative-strand RNA viruses, remain largely unexplored. Here, we employ a proteomics informed by transcriptomics approach to elucidate the cellular response of the Rhipicephalus microplus-derived BME/CTVM6 cell line to severe fever with thrombocytopenia syndrome virus (SFTSV) infection. We generate the de novo transcriptomes and proteomes of SFTSV- and mock-infected tick cells, identifying key host responses and regulatory pathways. Additionally, interactome analysis of the viral nucleoprotein (N) integrated host responses with viral replication and dsRNA-mediated gene silencing screen reveals two anti-SFTSV effectors: the N interacting RNA helicases DHX9 and UPF1. Collectively, our results provide insights into the antiviral responses of R. microplus vector cells and highlight critical SFTSV restriction factors, while enriching transcriptomic and proteomic resources for future research.

Original language	English
Article number	4732
Number of pages	16
Journal	Nature Communications
Volume	16
Issue number	1
Early online date	21 May 2025
DOIs	https://doi.org/10.1038/s41467-025-59565-w
Publication status	E-pub ahead of print - 21 May 2025

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

Access to Document

10.1038/s41467-025-59565-wLicence: CC BY

Final published version
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder
Final published version, 3.53 MBLicence: CC BY

Cite this

@article{c8c4f28273454ea88f5f0af5fa6f34d6,

title = "Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors",

abstract = "The increasing prevalence of tick-borne arboviral infections worldwide necessitates advanced control strategies, particularly those targeting vectors, to mitigate the disease burden. However, the cellular interactions between arboviruses and ticks, especially for negative-strand RNA viruses, remain largely unexplored. Here, we employ a proteomics informed by transcriptomics approach to elucidate the cellular response of the Rhipicephalus microplus-derived BME/CTVM6 cell line to severe fever with thrombocytopenia syndrome virus (SFTSV) infection. We generate the de novo transcriptomes and proteomes of SFTSV- and mock-infected tick cells, identifying key host responses and regulatory pathways. Additionally, interactome analysis of the viral nucleoprotein (N) integrated host responses with viral replication and dsRNA-mediated gene silencing screen reveals two anti-SFTSV effectors: the N interacting RNA helicases DHX9 and UPF1. Collectively, our results provide insights into the antiviral responses of R. microplus vector cells and highlight critical SFTSV restriction factors, while enriching transcriptomic and proteomic resources for future research.",

author = "Petit, \{Marine J.\} and Charlotte Flory and Quan Gu and Mazigh Fares and Douglas Lamont and Alan Score and Kelsey Davies and Lesley Bell-Sakyi and Pietro Scaturro and Benjamin Brennan and Alain Kohl",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2025.",

year = "2025",

month = may,

day = "21",

doi = "10.1038/s41467-025-59565-w",

language = "English",

volume = "16",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Research",

number = "1",

}

TY - JOUR

T1 - Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors

AU - Petit, Marine J.

AU - Flory, Charlotte

AU - Gu, Quan

AU - Fares, Mazigh

AU - Lamont, Douglas

AU - Score, Alan

AU - Davies, Kelsey

AU - Bell-Sakyi, Lesley

AU - Scaturro, Pietro

AU - Brennan, Benjamin

AU - Kohl, Alain

PY - 2025/5/21

Y1 - 2025/5/21

N2 - The increasing prevalence of tick-borne arboviral infections worldwide necessitates advanced control strategies, particularly those targeting vectors, to mitigate the disease burden. However, the cellular interactions between arboviruses and ticks, especially for negative-strand RNA viruses, remain largely unexplored. Here, we employ a proteomics informed by transcriptomics approach to elucidate the cellular response of the Rhipicephalus microplus-derived BME/CTVM6 cell line to severe fever with thrombocytopenia syndrome virus (SFTSV) infection. We generate the de novo transcriptomes and proteomes of SFTSV- and mock-infected tick cells, identifying key host responses and regulatory pathways. Additionally, interactome analysis of the viral nucleoprotein (N) integrated host responses with viral replication and dsRNA-mediated gene silencing screen reveals two anti-SFTSV effectors: the N interacting RNA helicases DHX9 and UPF1. Collectively, our results provide insights into the antiviral responses of R. microplus vector cells and highlight critical SFTSV restriction factors, while enriching transcriptomic and proteomic resources for future research.

AB - The increasing prevalence of tick-borne arboviral infections worldwide necessitates advanced control strategies, particularly those targeting vectors, to mitigate the disease burden. However, the cellular interactions between arboviruses and ticks, especially for negative-strand RNA viruses, remain largely unexplored. Here, we employ a proteomics informed by transcriptomics approach to elucidate the cellular response of the Rhipicephalus microplus-derived BME/CTVM6 cell line to severe fever with thrombocytopenia syndrome virus (SFTSV) infection. We generate the de novo transcriptomes and proteomes of SFTSV- and mock-infected tick cells, identifying key host responses and regulatory pathways. Additionally, interactome analysis of the viral nucleoprotein (N) integrated host responses with viral replication and dsRNA-mediated gene silencing screen reveals two anti-SFTSV effectors: the N interacting RNA helicases DHX9 and UPF1. Collectively, our results provide insights into the antiviral responses of R. microplus vector cells and highlight critical SFTSV restriction factors, while enriching transcriptomic and proteomic resources for future research.

UR - https://www.scopus.com/pages/publications/105005586405

U2 - 10.1038/s41467-025-59565-w

DO - 10.1038/s41467-025-59565-w

M3 - Article

C2 - 40399277

AN - SCOPUS:105005586405

SN - 2041-1723

VL - 16

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 4732

ER -

Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this