TY - JOUR
T1 - Multi-photon attenuation-compensated light-sheet fluorescence microscopy
AU - Veettikazhy, Madhu
AU - Nylk, Jonathan
AU - Gasparoli, Federico
AU - Escobet-Montalbán, Adrià
AU - Hansen, Anders Kragh
AU - Marti, Dominik
AU - Andersen, Peter Eskil
AU - Dholakia, Kishan
N1 - We thank the UK Engineering and Physical Sciences Research Council for funding (grants EP/P030017/1 and EP/R004854/1), the European Union’s Horizon 2020 Framework Programme (H2020) (675512, BE-OPTICAL), the Danish Council for Independent Research (DFF FTP grant 7017-00021), and the Otto Mønsted Foundation (grant 19-70-0109).
PY - 2020/5/15
Y1 - 2020/5/15
N2 - Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an increase in depth penetration for imaging. Here we show this promising approach may be implemented in multi-photon (two-photon) light-sheet fluorescence microscopy and, furthermore, can be achieved in a facile manner utilizing a graded neutral density filter, circumventing the need for complex beam shaping apparatus. A "gold standard" system utilizing a spatial light modulator for beam shaping is used to benchmark our implementation. The approach will open up enhanced depth penetration in light-sheet imaging to a wide range of end users.
AB - Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an increase in depth penetration for imaging. Here we show this promising approach may be implemented in multi-photon (two-photon) light-sheet fluorescence microscopy and, furthermore, can be achieved in a facile manner utilizing a graded neutral density filter, circumventing the need for complex beam shaping apparatus. A "gold standard" system utilizing a spatial light modulator for beam shaping is used to benchmark our implementation. The approach will open up enhanced depth penetration in light-sheet imaging to a wide range of end users.
KW - Cellular imaging
KW - Light-sheet microscopy
KW - Microscopy
KW - Multiphoton microscopy
KW - Optics and photonics
UR - http://www.scopus.com/inward/record.url?scp=85084786005&partnerID=8YFLogxK
U2 - 10.1038/s41598-020-64891-8
DO - 10.1038/s41598-020-64891-8
M3 - Article
C2 - 32415135
SN - 2045-2322
VL - 10
SP - 1
EP - 10
JO - Scientific Reports
JF - Scientific Reports
M1 - 8090
ER -