Multi-scale non-local means with shape prior for enhancement of cell membrane images

Cheng-Jin Du, Richard Tyson, Emil Rozbicki, Cornelis J. Weijer, Till Bretschneider

    Research output: Chapter in Book/Report/Conference proceedingConference contribution


    Light sheet microscopy allows rapid optical sectioning of whole chicken embryos during early development, visualizing cells through transgenic expression of a fluorescent cell membrane label. Low signal-to-noise ratios and large variability in intensity make segmentation of these images difficult, often resulting in discontinuous cell edges. We propose a multi-scale non-local means with shape prior (MS-NLM-SP) method for image enhancement, which takes into account cell-specific characteristics, such as size, geometry, and periodicity. Investigating theoretical connections between our approach and anisotropic diffusion reveals that the first step, detail-enhanced multi-scale representation, corresponds to selective backward diffusion, while the second step of MS-NLM-SP resembles anisotropic forward diffusion. Experimental results show that incorporating cell-specific features into non-local image denoising methods improves subsequent segmentation.

    Original languageEnglish
    Title of host publication2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014
    Subtitle of host publicationProceedings
    Number of pages4
    ISBN (Print)9781467319591
    Publication statusPublished - 2014
    Event2014 IEEE International Symposium on Biomedical Imaging - Renaissance Beijing Capital Hotel, Beijing, China
    Duration: 29 Apr 20142 May 2014


    Conference2014 IEEE International Symposium on Biomedical Imaging
    Abbreviated titleISBI 2014
    Internet address


    • Cell membrane
    • Image enhancement
    • Multi-scale representation
    • Non-local means
    • Shape prior

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Radiology Nuclear Medicine and imaging


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