Multimode fibre: Light-sheet microscopy at the tip of a needle

Martin Plöschner; Vera Kollarova; Zbynek Dostal; Jonathan Nylk; Thomas Barton-Owen; David E. K. Ferrier; Radim Chmelik; Kishan Dholakia; Tomas Cizmar

doi:10.1038/srep18050

Multimode fibre: Light-sheet microscopy at the tip of a needle

Martin Plöschner, Vera Kollarova, Zbynek Dostal, Jonathan Nylk, Thomas Barton-Owen, David E. K. Ferrier, Radim Chmelik, Kishan Dholakia, Tomas Cizmar (Lead / Corresponding author)

Physics

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50 Citations (Scopus)

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Abstract

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

Original language	English
Article number	18050
Pages (from-to)	1-7
Number of pages	7
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep18050
Publication status	Published - 14 Dec 2015

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10.1038/srep18050Licence: CC BY

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Cite this

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title = "Multimode fibre: Light-sheet microscopy at the tip of a needle",

abstract = "Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.",

author = "Martin Pl{\"o}schner and Vera Kollarova and Zbynek Dostal and Jonathan Nylk and Thomas Barton-Owen and Ferrier, {David E. K.} and Radim Chmelik and Kishan Dholakia and Tomas Cizmar",

note = "The authors would like to acknowledge support from the University of St Andrews, the University of Dundee and the Scottish University Physics Alliance (SUPA). We also thank the UK Engineering and Physics Sciences Research Council for funding under grant EP/J01771X/1. Finally, we would like to thank EXCELLENT TEAMS (CZ.1.07/2.3.00/30.0005) from European Social Fund and CEITEC - Central European Institute of Technology (CZ.1.05/1.1.00/02.0068) from European Regional Development Fund for support.",

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doi = "10.1038/srep18050",

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AU - Plöschner, Martin

AU - Kollarova, Vera

AU - Dostal, Zbynek

AU - Nylk, Jonathan

AU - Barton-Owen, Thomas

AU - Ferrier, David E. K.

AU - Chmelik, Radim

AU - Dholakia, Kishan

AU - Cizmar, Tomas

N1 - The authors would like to acknowledge support from the University of St Andrews, the University of Dundee and the Scottish University Physics Alliance (SUPA). We also thank the UK Engineering and Physics Sciences Research Council for funding under grant EP/J01771X/1. Finally, we would like to thank EXCELLENT TEAMS (CZ.1.07/2.3.00/30.0005) from European Social Fund and CEITEC - Central European Institute of Technology (CZ.1.05/1.1.00/02.0068) from European Regional Development Fund for support.

PY - 2015/12/14

Y1 - 2015/12/14

N2 - Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

AB - Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

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JO - Scientific Reports

JF - Scientific Reports

M1 - 18050

ER -

Multimode fibre: Light-sheet microscopy at the tip of a needle

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